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Assay Detail

CHEMBL5100169

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in Sf9 insect cells using Z-Lys(Ac)-AMC as fluorogenic substrate incubated for 90 mins by microplate reader analysis
27
Total Activities
24
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 6 (CHEMBL1865)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of Fluorinated Peptoid-Based Histone Deacetylase (HDAC) Inhibitors for Therapy-Resistant Acute Leukemia.
Reßing N, Schliehe-Diecks J, Watson PR, Sönnichsen M, Cragin AD, Schöler A, Yang J, Schäker-Hübner L, Borkhardt A, Christianson DW, Bhatia S, Hansen FK.
J Med Chem (2022) 65 : 15457 -15472
PubMed 36351184 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 7.56 8.00
IC5 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5187433 3 8.00
CHEMBL4170198 1 7.96
CHEMBL5189808 1 7.89
CHEMBL2018302 TUBASTATIN A 1 7.85
CHEMBL1213492 GIVINOSTAT 4.0 1 7.85
CHEMBL5199849 1 7.80
CHEMBL5201168 1 7.77
CHEMBL5184839 1 7.75
CHEMBL5190554 1 7.75
CHEMBL5209024 1 7.70
CHEMBL5180418 1 7.70
CHEMBL5183285 1 7.70
CHEMBL5197066 1 7.68
CHEMBL5194945 1 7.62
CHEMBL5189667 1 7.58
CHEMBL5188109 1 7.57
CHEMBL5204510 1 7.57
CHEMBL5187865 1 7.57
CHEMBL5188237 1 7.42
CHEMBL4173361 1 7.40
CHEMBL5170634 2 7.38
CHEMBL98 VORINOSTAT 4.0 1 7.38
CHEMBL5187265 1 7.29
CHEMBL4066043 1 7.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5187433 IC50 = 10.0 nM 8.00
CHEMBL4170198 IC50 = 11.0 nM 7.96
CHEMBL5189808 IC50 = 13.0 nM 7.89
CHEMBL2018302 TUBASTATIN A IC50 = 14.0 nM 7.85
CHEMBL1213492 GIVINOSTAT IC50 = 14.0 nM 7.85
CHEMBL5199849 IC50 = 16.0 nM 7.80
CHEMBL5201168 IC50 = 17.0 nM 7.77
CHEMBL5184839 IC50 = 18.0 nM 7.75
CHEMBL5190554 IC50 = 18.0 nM 7.75
CHEMBL5183285 IC50 = 20.0 nM 7.70
CHEMBL5209024 IC50 = 20.0 nM 7.70
CHEMBL5180418 IC50 = 20.0 nM 7.70
CHEMBL5197066 IC50 = 21.0 nM 7.68
CHEMBL5194945 IC50 = 24.0 nM 7.62
CHEMBL5189667 IC50 = 26.0 nM 7.58
CHEMBL5204510 IC50 = 27.0 nM 7.57
CHEMBL5188109 IC50 = 27.0 nM 7.57
CHEMBL5187865 IC50 = 27.0 nM 7.57
CHEMBL5188237 IC50 = 38.0 nM 7.42
CHEMBL4173361 IC50 = 40.0 nM 7.40
CHEMBL5170634 IC50 = 42.0 nM 7.38
CHEMBL98 VORINOSTAT IC50 = 42.0 nM 7.38
CHEMBL5187265 IC50 = 51.0 nM 7.29
CHEMBL4066043 IC50 = 85.0 nM 7.07
CHEMBL5187433 IC50 = 1880.0 nM 5.73
CHEMBL5187433 IC5 = 1.88 uM -
CHEMBL5170634 IC50 > 10000.0 nM -