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Assay Detail

CHEMBL5106115

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]PSB-603 from human adenosine A2B receptor expressed in CHO cell membrane and measured after 0.5 hrs by radioligand displacement assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Subcellular Cell membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2b (CHEMBL255)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of subtype-selective covalent ligands for the adenosine A<sub>2B</sub> receptor by tuning the reactive group.
Beerkens BLH, Wang X, Avgeropoulou M, Adistia LN, van Veldhoven JPD, Jespers W, Liu R, Heitman LH, IJzerman AP, van der Es D.
RSC Med Chem (2022) 13 : 850 -856
PubMed 35923720 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 7.13 8.67

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5207498 1 8.67
CHEMBL5207290 1 8.10
CHEMBL5205220 1 7.93
CHEMBL5196082 1 7.62
CHEMBL5176227 1 7.31
CHEMBL5172513 1 7.22
CHEMBL5208506 1 6.96
CHEMBL5195884 1 6.88
CHEMBL5185103 1 6.85
CHEMBL8565 1 6.71
CHEMBL5172969 1 6.25
CHEMBL5175698 1 6.21
CHEMBL5198830 1 6.02

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5207498 Ki = 2.138 nM 8.67
CHEMBL5207290 Ki = 7.943 nM 8.10
CHEMBL5205220 Ki = 11.75 nM 7.93
CHEMBL5196082 Ki = 23.99 nM 7.62
CHEMBL5176227 Ki = 48.98 nM 7.31
CHEMBL5172513 Ki = 60.26 nM 7.22
CHEMBL5208506 Ki = 109.65 nM 6.96
CHEMBL5195884 Ki = 131.83 nM 6.88
CHEMBL5185103 Ki = 141.25 nM 6.85
CHEMBL8565 Ki = 194.98 nM 6.71
CHEMBL5172969 Ki = 562.34 nM 6.25
CHEMBL5175698 Ki = 616.6 nM 6.21
CHEMBL5198830 Ki = 954.99 nM 6.02