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Assay Detail

CHEMBL5108908

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Irreversible inhibition of recombinant Streptococcus pyogenes sortase A deltaN81 expressed in Escherichia coli BL21(DE3) cells using Abz-LPATG-Dnp as substrate assessed as inactivation constant preincubated for 60 mins followed by substrate addition and measured every 1 min for 15 mins by fluorescence intensity based assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Streptococcus pyogenes
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Covalent sortase A inhibitor ML346 prevents <i>Staphylococcus aureus</i> infection of <i>Galleria mellonella</i>.
Guan XN, Zhang T, Yang T, Dong Z, Yang S, Lan L, Gan J, Yang CG.
RSC Med Chem (2022) 13 : 138 -149
PubMed 35308030 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 1 6.16 6.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5174805 1 6.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5174805 Ki = 686.0 nM 6.16