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Assay Detail

CHEMBL5110447

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TRKA G667A mutant (unknown origin) using TK-sub-biotin peptide as substrate incubated for 30 mins followed by treated with ATP and substrate for 40 mins by FRET-based-Z'-lyte kinase assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of 3-pyrazolyl-substituted pyrazolo[1,5-a]pyrimidine derivatives as potent TRK inhibitors to overcome clinically acquired resistance.
Gong Y, Wu FX, Wang MS, Xu HC, Zhuo LS, Yang GF, Huang W.
Eur J Med Chem (2022) 241 : 114654 -114654
PubMed 35961071 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 8.08 8.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5195375 1 8.64
CHEMBL5198983 1 8.46
CHEMBL5170096 1 8.29
CHEMBL5188045 1 8.28
CHEMBL5169970 1 8.18
CHEMBL5170464 1 8.12
CHEMBL5205989 1 8.12
CHEMBL5189971 1 8.11
CHEMBL5173256 1 8.10
CHEMBL5193919 1 8.07
CHEMBL5196651 1 8.06
CHEMBL4297627 SELITRECTINIB 2.0 1 7.90
CHEMBL5175608 1 7.82
CHEMBL5184432 1 7.81
CHEMBL5170412 1 7.80
CHEMBL3889654 LAROTRECTINIB 4.0 1 7.49

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5195375 IC50 = 2.3 nM 8.64
CHEMBL5198983 IC50 = 3.5 nM 8.46
CHEMBL5170096 IC50 = 5.1 nM 8.29
CHEMBL5188045 IC50 = 5.2 nM 8.28
CHEMBL5169970 IC50 = 6.6 nM 8.18
CHEMBL5170464 IC50 = 7.5 nM 8.12
CHEMBL5205989 IC50 = 7.5 nM 8.12
CHEMBL5189971 IC50 = 7.7 nM 8.11
CHEMBL5173256 IC50 = 8.0 nM 8.10
CHEMBL5193919 IC50 = 8.6 nM 8.07
CHEMBL5196651 IC50 = 8.7 nM 8.06
CHEMBL4297627 SELITRECTINIB IC50 = 12.6 nM 7.90
CHEMBL5175608 IC50 = 15.3 nM 7.82
CHEMBL5184432 IC50 = 15.4 nM 7.81
CHEMBL5170412 IC50 = 16.0 nM 7.80
CHEMBL3889654 LAROTRECTINIB IC50 = 32.2 nM 7.49