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Assay Detail

CHEMBL5111430

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against MHV-nLuc infected in DBT cells assessed as effect on cell viability by LDH assay
30
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Murine hepatitis virus
Cell Type Astrocytoma delayed brain tumor (DBT) cell
Confidence 1 — Organism
Curated By Autocuration

Target

Murine hepatitis virus (CHEMBL613733)
Type ORGANISM
Organism Murine hepatitis virus

Publication

Identification and Utilization of a Chemical Probe to Interrogate the Roles of PIKfyve in the Lifecycle of β-Coronaviruses.
Drewry DH, Potjewyd FM, Bayati A, Smith JL, Dickmander RJ, Howell S, Taft-Benz S, Min SM, Hossain MA, Heise M, McPherson PS, Moorman NJ, Axtman AD.
J Med Chem (2022) 65 : 12860 -12882
PubMed 36111834 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 6.33 7.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4297643 APILIMOD 2.0 2 7.80
CHEMBL5174830 2 7.63
CHEMBL4303292 2 7.39
CHEMBL5171753 2 7.15
CHEMBL5189479 2 7.13
CHEMBL2178735 2 6.60
CHEMBL5190524 2 6.31
CHEMBL5188159 2 6.18
CHEMBL5207387 2 6.15
CHEMBL5190724 2 5.75
CHEMBL5171207 2 5.72
CHEMBL5200109 2 5.27
CHEMBL5192827 2 5.09
CHEMBL5202422 2 5.07
CHEMBL5199026 2 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4297643 APILIMOD IC50 = 15.85 nM 7.80
CHEMBL4297643 APILIMOD IC50 = 15.7 nM 7.80
CHEMBL5174830 IC50 = 23.44 nM 7.63
CHEMBL5174830 IC50 = 23.5 nM 7.63
CHEMBL4303292 IC50 = 40.4 nM 7.39
CHEMBL4303292 IC50 = 40.74 nM 7.39
CHEMBL5171753 IC50 = 70.79 nM 7.15
CHEMBL5171753 IC50 = 71.0 nM 7.15
CHEMBL5189479 IC50 = 74.13 nM 7.13
CHEMBL5189479 IC50 = 74.0 nM 7.13
CHEMBL2178735 IC50 = 250.0 nM 6.60
CHEMBL2178735 IC50 = 251.19 nM 6.60
CHEMBL5190524 IC50 = 485.0 nM 6.31
CHEMBL5190524 IC50 = 489.78 nM 6.31
CHEMBL5188159 IC50 = 660.69 nM 6.18
CHEMBL5188159 IC50 = 666.0 nM 6.18
CHEMBL5207387 IC50 = 713.0 nM 6.15
CHEMBL5207387 IC50 = 707.95 nM 6.15
CHEMBL5190724 IC50 = 1800.0 nM 5.75
CHEMBL5190724 IC50 = 1778.28 nM 5.75
CHEMBL5171207 IC50 = 1920.0 nM 5.72
CHEMBL5171207 IC50 = 1905.46 nM 5.72
CHEMBL5200109 IC50 = 5370.32 nM 5.27
CHEMBL5200109 IC50 = 5350.0 nM 5.27
CHEMBL5192827 IC50 = 8128.31 nM 5.09
CHEMBL5192827 IC50 = 8050.0 nM 5.09
CHEMBL5202422 IC50 = 8511.38 nM 5.07
CHEMBL5202422 IC50 = 8470.0 nM 5.07
CHEMBL5199026 IC50 = 10000.0 nM 5.00
CHEMBL5199026 IC50 > 10000.0 nM -