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Assay Detail

CHEMBL5111971

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human N-terminal GST-fusion tagged DHODH (31 to 395 residues) expressed in Escherichia coli BL21-GOLD (DE3) using dihydroorotate as substrate preincubated for 5 mins followed by substrate addition measured after 5 mins by DCIP assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dihydroorotate dehydrogenase (quinone), mitochondrial (CHEMBL1966)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Targeting Acute Myelogenous Leukemia Using Potent Human Dihydroorotate Dehydrogenase Inhibitors Based on the 2-Hydroxypyrazolo[1,5-<i>a</i>]pyridine Scaffold: SAR of the Aryloxyaryl Moiety.
Sainas S, Giorgis M, Circosta P, Poli G, Alberti M, Passoni A, Gaidano V, Pippione AC, Vitale N, Bonanni D, Rolando B, Cignetti A, Ramondetti C, Lanno A, Ferraris DM, Canepa B, Buccinnà B, Piccinini M, Rizzi M, Saglio G, Al-Karadaghi S, Boschi D, Miggiano R, Tuccinardi T, Lolli ML.
J Med Chem (2022) 65 : 12701 -12724
PubMed 36162075 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.27 8.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5192490 1 8.14
CHEMBL5196292 1 7.75
CHEMBL4165815 1 7.30
CHEMBL5183718 1 7.16
CHEMBL5209405 1 6.94
CHEMBL5182435 1 6.40
CHEMBL5190641 1 5.66
CHEMBL5181901 1 5.66
CHEMBL5172821 1 5.55
CHEMBL5178813 1 5.41
CHEMBL5173387 1 5.28
CHEMBL5193995 1 5.16
CHEMBL5188838 1 5.14
CHEMBL5180390 1 -
CHEMBL5181455 1 -
CHEMBL5185261 1 -
CHEMBL5203155 1 -
CHEMBL5169905 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5192490 IC50 = 7.2 nM 8.14
CHEMBL5196292 IC50 = 18.0 nM 7.75
CHEMBL4165815 IC50 = 50.0 nM 7.30
CHEMBL5183718 IC50 = 70.0 nM 7.16
CHEMBL5209405 IC50 = 114.0 nM 6.94
CHEMBL5182435 IC50 = 400.0 nM 6.40
CHEMBL5190641 IC50 = 2209.0 nM 5.66
CHEMBL5181901 IC50 = 2200.0 nM 5.66
CHEMBL5172821 IC50 = 2800.0 nM 5.55
CHEMBL5178813 IC50 = 3900.0 nM 5.41
CHEMBL5173387 IC50 = 5200.0 nM 5.28
CHEMBL5193995 IC50 = 7000.0 nM 5.16
CHEMBL5188838 IC50 = 7200.0 nM 5.14
CHEMBL5180390 IC50 > 10000.0 nM -
CHEMBL5181455 IC50 > 10000.0 nM -
CHEMBL5185261 IC50 > 10000.0 nM -
CHEMBL5203155 IC50 > 10000.0 nM -
CHEMBL5169905 IC50 > 10000.0 nM -