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Assay Detail

CHEMBL5111997

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to wild type A3R (unknown origin) expressed in CHO-K1 cells assessed as equilibrium binding affinity constant by nanoBRET assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Flp-In-CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A3 (CHEMBL256)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Dual A1/A3 Adenosine Receptor Antagonists: Binding Kinetics and Structure-Activity Relationship Studies Using Mutagenesis and Alchemical Binding Free Energy Calculations.
Stampelou M, Suchankova A, Tzortzini E, Dhingra L, Barkan K, Lougiakis N, Marakos P, Pouli N, Ladds G, Kolocouris A.
J Med Chem (2022) 65 : 13305 -13327
PubMed 36173355 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 17 7.00 9.94

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5201810 1 9.94
CHEMBL5181728 1 8.01
CHEMBL5180268 1 7.36
CHEMBL3929812 1 7.26
CHEMBL88147 1 7.19
CHEMBL5174600 1 7.19
CHEMBL5185003 1 7.07
CHEMBL3929143 1 7.00
CHEMBL5189274 1 6.95
CHEMBL5196781 1 6.88
CHEMBL464859 NECA 1 6.72
CHEMBL5186062 1 6.63
CHEMBL5180010 1 6.60
CHEMBL5189470 1 6.33
CHEMBL5173159 1 6.22
CHEMBL3927012 1 6.20
CHEMBL5187501 1 5.49

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5201810 Ki = 0.1148 nM 9.94
CHEMBL5181728 Ki = 9.772 nM 8.01
CHEMBL5180268 Ki = 43.65 nM 7.36
CHEMBL3929812 Ki = 54.95 nM 7.26
CHEMBL88147 Ki = 64.57 nM 7.19
CHEMBL5174600 Ki = 64.57 nM 7.19
CHEMBL5185003 Ki = 85.11 nM 7.07
CHEMBL3929143 Ki = 100.0 nM 7.00
CHEMBL5189274 Ki = 112.2 nM 6.95
CHEMBL5196781 Ki = 131.83 nM 6.88
CHEMBL464859 NECA Ki = 190.55 nM 6.72
CHEMBL5186062 Ki = 234.42 nM 6.63
CHEMBL5180010 Ki = 251.19 nM 6.60
CHEMBL5189470 Ki = 467.74 nM 6.33
CHEMBL5173159 Ki = 602.56 nM 6.22
CHEMBL3927012 Ki = 630.96 nM 6.20
CHEMBL5187501 Ki = 3235.94 nM 5.49