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Assay Detail

CHEMBL5113063

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Binding
Inverse agonist activity at PPARgamma in human RT112/84-FABP4 cells assessed as reduction in PPARgamma transactivation measured by Nanoluciferase reporter gene assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type RT112/84
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor gamma (CHEMBL235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Structure-Based Design of Potent Covalent PPARγ Inverse-Agonists <b>BAY-4931</b> and <b>BAY-0069</b>.
Orsi DL, Pook E, Bräuer N, Friberg A, Lienau P, Lemke CT, Stellfeld T, Brüggemeier U, Pütter V, Meyer H, Baco M, Tang S, Cherniack AD, Westlake L, Bender SA, Kocak M, Strathdee CA, Meyerson M, Eis K, Goldstein JT.
J Med Chem (2022) 65 : 14843 -14863
PubMed 36270630 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 8.05 9.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5191837 1 9.80
CHEMBL5207130 1 9.77
CHEMBL5187164 1 9.66
CHEMBL5179281 1 9.64
CHEMBL5206512 1 9.60
CHEMBL5207464 1 9.54
CHEMBL5204936 1 9.49
CHEMBL5201278 1 9.24
CHEMBL5184027 1 8.80
CHEMBL5199152 1 8.48
CHEMBL5206276 1 8.40
CHEMBL5183657 1 8.36
CHEMBL5173312 1 8.07
CHEMBL5174641 1 7.28
CHEMBL5201306 1 6.71
CHEMBL2324298 1 6.32
CHEMBL5186962 1 5.89
CHEMBL5182665 1 5.75
CHEMBL5179111 1 5.24
CHEMBL5172001 1 5.03
CHEMBL5170260 1 -
CHEMBL5200843 1 -
CHEMBL5203129 1 -
CHEMBL5171685 1 -
CHEMBL5169731 1 -
CHEMBL2447934 1 -
CHEMBL5201597 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5191837 IC50 = 0.16 nM 9.80
CHEMBL5207130 IC50 = 0.17 nM 9.77
CHEMBL5187164 IC50 = 0.22 nM 9.66
CHEMBL5179281 IC50 = 0.23 nM 9.64
CHEMBL5206512 IC50 = 0.25 nM 9.60
CHEMBL5207464 IC50 = 0.29 nM 9.54
CHEMBL5204936 IC50 = 0.32 nM 9.49
CHEMBL5201278 IC50 = 0.58 nM 9.24
CHEMBL5184027 IC50 = 1.6 nM 8.80
CHEMBL5199152 IC50 = 3.3 nM 8.48
CHEMBL5206276 IC50 = 4.0 nM 8.40
CHEMBL5183657 IC50 = 4.4 nM 8.36
CHEMBL5173312 IC50 = 8.6 nM 8.07
CHEMBL5174641 IC50 = 52.0 nM 7.28
CHEMBL5201306 IC50 = 195.0 nM 6.71
CHEMBL2324298 IC50 = 480.0 nM 6.32
CHEMBL5186962 IC50 = 1300.0 nM 5.89
CHEMBL5182665 IC50 = 1800.0 nM 5.75
CHEMBL5179111 IC50 = 5750.0 nM 5.24
CHEMBL5172001 IC50 = 9350.0 nM 5.03
CHEMBL5170260 IC50 > 50000.0 nM -
CHEMBL5200843 IC50 > 50000.0 nM -
CHEMBL5203129 IC50 > 33000.0 nM -
CHEMBL5171685 IC50 > 50000.0 nM -
CHEMBL5169731 IC50 > 50000.0 nM -
CHEMBL2447934 IC50 > 50000.0 nM -
CHEMBL5201597 IC50 > 50000.0 nM -