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Assay Detail

CHEMBL5114469

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GAT-mediated [3H]-GABA uptake in rat forebrain synaptosomes preincubated for 8 mins followed by [3H]-GABA addition measured after 8 mins by scintillation counting method
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Forebrain
Subcellular Synaptosomes
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Nipecotic acid as potential lead molecule for the development of GABA uptake inhibitors; structural insights and design strategies.
Singh K, Kumar P, Bhatia R, Mehta V, Kumar B, Akhtar MJ.
Eur J Med Chem (2022) 234 : 114269 -114269
PubMed 35306287 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 7.15 7.21

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5170782 1 7.21
CHEMBL1695 TIAGABINE HYDROCHLORIDE 4.0 1 7.17
CHEMBL5204409 1 7.11
CHEMBL5177063 1 7.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5170782 IC50 = 61.0 nM 7.21
CHEMBL1695 TIAGABINE HYDROCHLORIDE IC50 = 67.0 nM 7.17
CHEMBL5204409 IC50 = 78.0 nM 7.11
CHEMBL5177063 IC50 = 80.0 nM 7.10