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Assay Detail

CHEMBL5118400

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Binding
Antagonist activity at human P2X3R expressed in HEK293 cells assessed as reduction in alphabeta-MeATP-induced intracellular Ca2+ influx pretreated with compound followed by alphabeta-MeATP addition and measured by Fluo-4 dye fluorescence analysis
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 3 (CHEMBL2998)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

AI-based prediction of new binding site and virtual screening for the discovery of novel P2X3 receptor antagonists.
Kang KM, Lee I, Nam H, Kim YC.
Eur J Med Chem (2022) 240 : 114556 -114556
PubMed 35849939 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 5.74 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5186232 1 8.52
CHEMBL5084939 1 8.38
CHEMBL5095224 ELIAPIXANT 2.0 1 8.10
CHEMBL5207245 1 7.60
CHEMBL3716057 GEFAPIXANT 4.0 1 7.35
CHEMBL5170554 1 5.71
CHEMBL5178333 1 5.51
CHEMBL5178054 1 5.46
CHEMBL5186736 1 5.25
CHEMBL5182519 1 5.07
CHEMBL5199426 1 5.06
CHEMBL5201961 1 5.01
CHEMBL5175267 1 4.98
CHEMBL5191901 1 4.96
CHEMBL5194075 1 4.94
CHEMBL5182831 1 4.87
CHEMBL5194597 1 4.86
CHEMBL5196092 1 4.81
CHEMBL5193198 1 4.74
CHEMBL5192824 1 4.72
CHEMBL5185378 1 4.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5186232 IC50 = 3.0 nM 8.52
CHEMBL5084939 IC50 = 4.2 nM 8.38
CHEMBL5095224 ELIAPIXANT IC50 = 8.0 nM 8.10
CHEMBL5207245 IC50 = 25.0 nM 7.60
CHEMBL3716057 GEFAPIXANT IC50 = 45.0 nM 7.35
CHEMBL5170554 IC50 = 1930.0 nM 5.71
CHEMBL5178333 IC50 = 3070.0 nM 5.51
CHEMBL5178054 IC50 = 3430.0 nM 5.46
CHEMBL5186736 IC50 = 5610.0 nM 5.25
CHEMBL5182519 IC50 = 8560.0 nM 5.07
CHEMBL5199426 IC50 = 8720.0 nM 5.06
CHEMBL5201961 IC50 = 9760.0 nM 5.01
CHEMBL5175267 IC50 = 10400.0 nM 4.98
CHEMBL5191901 IC50 = 10900.0 nM 4.96
CHEMBL5194075 IC50 = 11500.0 nM 4.94
CHEMBL5182831 IC50 = 13400.0 nM 4.87
CHEMBL5194597 IC50 = 13800.0 nM 4.86
CHEMBL5196092 IC50 = 15500.0 nM 4.81
CHEMBL5193198 IC50 = 18400.0 nM 4.74
CHEMBL5192824 IC50 = 19000.0 nM 4.72
CHEMBL5185378 IC50 = 21900.0 nM 4.66