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Assay Detail

CHEMBL5118486

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to NME4 in human MCF7 cell lysate by pull down assay based LC-MS/MS analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MCF7
Subcellular Lysate
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nucleoside diphosphate kinase, mitochondrial (CHEMBL4488)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of hetero-triaryls as a new chemotype for subtype-selective and potent Sirt5 inhibition.
Glas C, Naydenova E, Lechner S, Wössner N, Yang L, Dietschreit JCB, Sun H, Jung M, Kuster B, Ochsenfeld C, Bracher F.
Eur J Med Chem (2022) 240 : 114594 -114594
PubMed 35853430 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 4.76 5.11

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5204662 1 5.11
CHEMBL5176252 1 4.84
CHEMBL1201346 BALSALAZIDE 4.0 1 4.34

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5204662 EC50 = 7800.0 nM 5.11
CHEMBL5176252 EC50 = 14500.0 nM 4.84
CHEMBL1201346 BALSALAZIDE EC50 = 45800.0 nM 4.34