Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5120629

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Toxicity
Inhibition of JAK2 signaling pathway in human CHEP cells assessed as inhibition of EPO-induced cell survival preincubated for 1 hr followed by EPO addition and measured after 48 hrs by CellTiter-Glo luminescent assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Toxicity
Organism Homo sapiens
Cell Type CHEP
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK2 (CHEMBL2971)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of Pyrimidine Compounds as Potent JAK1 Inhibitors and the Discovery of R507 as a Clinical Candidate.
Chen Y, Li H, Yen R, Heckrodt TJ, McMurtrie D, Singh R, Taylor V, Masuda ES, Park G, Payan DG.
ACS Med Chem Lett (2022) 13 : 1805 -1811
PubMed 36385926 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.54 7.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5187209 1 7.38
CHEMBL5192889 1 7.09
CHEMBL5205935 1 7.08
CHEMBL5198389 1 6.88
CHEMBL5170161 1 6.77
CHEMBL5209379 1 6.76
CHEMBL5184050 1 6.45
CHEMBL4594441 IFIDANCITINIB 2.0 1 6.31
CHEMBL5203646 1 6.30
CHEMBL5188333 1 5.96
CHEMBL5173045 1 5.77
CHEMBL5192063 1 5.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5187209 IC50 = 42.0 nM 7.38
CHEMBL5192889 IC50 = 81.0 nM 7.09
CHEMBL5205935 IC50 = 84.0 nM 7.08
CHEMBL5198389 IC50 = 132.0 nM 6.88
CHEMBL5170161 IC50 = 170.0 nM 6.77
CHEMBL5209379 IC50 = 173.0 nM 6.76
CHEMBL5184050 IC50 = 354.0 nM 6.45
CHEMBL4594441 IFIDANCITINIB IC50 = 490.0 nM 6.31
CHEMBL5203646 IC50 = 503.0 nM 6.30
CHEMBL5188333 IC50 = 1110.0 nM 5.96
CHEMBL5173045 IC50 = 1689.0 nM 5.77
CHEMBL5192063 IC50 = 1976.0 nM 5.70