Assay Detail
Binding
CHEMBL5123586
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Inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Amplex red and horseradish peroxidase based fluorescence assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
New salicylic acid derivatives, double inhibitors of glycolate oxidase and lactate dehydrogenase, as effective agents decreasing oxalate production.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 5.20 | 5.82 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5180187 | — | — | 1 | 5.82 |
| CHEMBL5170581 | — | — | 1 | 5.70 |
| CHEMBL5204083 | — | — | 1 | 5.62 |
| CHEMBL5182027 | — | — | 1 | 5.38 |
| CHEMBL5197026 | — | — | 1 | 4.99 |
| CHEMBL5177237 | — | — | 1 | 4.78 |
| CHEMBL5190607 | — | — | 1 | 4.76 |
| CHEMBL4247590 | — | — | 1 | 4.55 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5180187 | — | IC50 | = | 1500.0 | nM | 5.82 |
| CHEMBL5170581 | — | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL5204083 | — | IC50 | = | 2400.0 | nM | 5.62 |
| CHEMBL5182027 | — | IC50 | = | 4200.0 | nM | 5.38 |
| CHEMBL5197026 | — | IC50 | = | 10300.0 | nM | 4.99 |
| CHEMBL5177237 | — | IC50 | = | 16800.0 | nM | 4.78 |
| CHEMBL5190607 | — | IC50 | = | 17300.0 | nM | 4.76 |
| CHEMBL4247590 | — | IC50 | = | 28200.0 | nM | 4.55 |