Assay Detail
Binding
CHEMBL5123594
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Non-competitive mixed inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as inhibition constant using varying concentration of glycolate as substrate measured every 60 sec for 15 mins by Amplex red and horseradish peroxidase based fluorescence assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
New salicylic acid derivatives, double inhibitors of glycolate oxidase and lactate dehydrogenase, as effective agents decreasing oxalate production.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 4 | 5.24 | 5.96 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5182027 | — | — | 1 | 5.96 |
| CHEMBL5180187 | — | — | 1 | 5.64 |
| CHEMBL4247590 | — | — | 1 | 4.74 |
| CHEMBL5190607 | — | — | 1 | 4.61 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5182027 | — | Ki | = | 1100.0 | nM | 5.96 |
| CHEMBL5180187 | — | Ki | = | 2300.0 | nM | 5.64 |
| CHEMBL4247590 | — | Ki | = | 18200.0 | nM | 4.74 |
| CHEMBL5190607 | — | Ki | = | 24700.0 | nM | 4.61 |