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Assay Detail

CHEMBL5123647

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PARP-1 (unknown origin) using biotinylated NAD+ as substrate incubated for 45 mins in the presence of deoxy-oligonucleotide by microplate reader method relative to control
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Poly [ADP-ribose] polymerase 1 (CHEMBL3105)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Rational design, synthesis and biological evaluation of dual PARP-1/2 and TNKS1/2 inhibitors for cancer therapy.
Xu Y, Wu H, Huang L, Zhai B, Li X, Xu S, Wu X, Zhu Q, Xu Q.
Eur J Med Chem (2022) 237 : 114417 -114417
PubMed 35504210 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 8.99 9.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5176874 1 9.60
CHEMBL5174780 1 9.31
CHEMBL5179949 1 9.01
CHEMBL521686 OLAPARIB 4.0 1 9.00
CHEMBL5189723 1 8.81
CHEMBL5172909 1 8.79
CHEMBL5174447 1 8.39
CHEMBL5197830 1 -
CHEMBL5185706 1 -
CHEMBL5191145 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5176874 IC50 = 0.25 nM 9.60
CHEMBL5174780 IC50 = 0.49 nM 9.31
CHEMBL5179949 IC50 = 0.98 nM 9.01
CHEMBL521686 OLAPARIB IC50 = 1.0 nM 9.00
CHEMBL5189723 IC50 = 1.54 nM 8.81
CHEMBL5172909 IC50 = 1.62 nM 8.79
CHEMBL5174447 IC50 = 4.08 nM 8.39
CHEMBL5197830 IC50 - -
CHEMBL5185706 IC50 - -
CHEMBL5191145 IC50 - -