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Assay Detail

CHEMBL5124546

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Immunosuppressive activity in BALB/c mouse splenocytes assessed as reduction in LPS-induced mouse B-lymphocytes proliferation incubated for 8 hrs by beta scintillation counting based [3H]thymidine incorporation assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type B-cell
Confidence 1 — Organism
Curated By Autocuration

Target

B-cell line (CHEMBL614724)
Type CELL-LINE
Organism Mus musculus

Publication

Dolabrane-Type Diterpenoids with Immunosuppressive Activity from <i>Koilodepas hainanense</i>.
Ren Y, Tong X, Zhao Y, He SJ, Fan YY, Yue JM.
J Nat Prod (2022) 85 : 1581 -1590
PubMed 35678710 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 5.69 6.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5202789 1 6.54
CHEMBL160 CYCLOSPORINE 4.0 1 6.13
CHEMBL5205000 1 5.65
CHEMBL517524 1 5.52
CHEMBL5172008 1 5.33
CHEMBL5183271 1 4.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5202789 IC50 = 290.0 nM 6.54
CHEMBL160 CYCLOSPORINE IC50 = 740.0 nM 6.13
CHEMBL5205000 IC50 = 2240.0 nM 5.65
CHEMBL517524 IC50 = 3050.0 nM 5.52
CHEMBL5172008 IC50 = 4720.0 nM 5.33
CHEMBL5183271 IC50 = 10250.0 nM 4.99