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Assay Detail

CHEMBL5124628

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Aurora A (122 to 403 residues) (unknown origin) expressed in Escherichia coli Rosetta 2(DE3) assessed as reduction in TACC3 phosphorylation at Ser 558 residue using TACC3 (519 to 838 residues) as substrate measured after 30 mins by Western blot analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aurora kinase A (CHEMBL4722)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification and Characterization of Natural and Semisynthetic Quinones as Aurora Kinase Inhibitors.
Furqan M, Fayyaz A, Firdous F, Raza H, Bilal A, Saleem RSZ, Shahzad-Ul-Hussan S, Wang D, Youssef FS, Al Musayeib NM, Ashour ML, Hussain H, Faisal A.
J Nat Prod (2022) 85 : 1503 -1513
PubMed 35687347 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.05 7.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL274056 NAPHTHAZALIN 1 7.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL274056 NAPHTHAZALIN IC50 = 90.0 nM 7.05