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Assay Detail

CHEMBL5125358

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal His6-tagged recombinant full length human PI3Kdelta expressed in baculovirus infected Sf21 insect cells incubated for 30 mins by HTRF assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery, Optimization, and Evaluation of Potent and Selective PI3Kδ-γ Dual Inhibitors for the Treatment of B-cell Malignancies.
Liu K, Zheng W, Chen Y, Tang M, Li D, Deng D, Yang T, Zhang C, Liu J, Yuan X, Shi M, Li X, Guo Y, Zhou Y, Zhao M, Chen L.
J Med Chem (2022) 65 : 9893 -9917
PubMed 35831917 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.67 8.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3039502 DUVELISIB 4.0 1 8.60
CHEMBL5178168 1 6.22
CHEMBL5179238 1 6.00
CHEMBL5203809 1 5.70
CHEMBL5182396 1 5.52
CHEMBL5200762 1 4.55
CHEMBL5182539 1 4.39
CHEMBL5189303 1 4.38

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3039502 DUVELISIB IC50 = 2.5 nM 8.60
CHEMBL5178168 IC50 = 600.0 nM 6.22
CHEMBL5179238 IC50 = 1000.0 nM 6.00
CHEMBL5203809 IC50 = 2000.0 nM 5.70
CHEMBL5182396 IC50 = 3000.0 nM 5.52
CHEMBL5200762 IC50 = 28000.0 nM 4.55
CHEMBL5182539 IC50 = 41000.0 nM 4.39
CHEMBL5189303 IC50 = 42000.0 nM 4.38