Assay Detail
Binding
CHEMBL5127927
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant KDM1A (unknown origin) expressed in Escherichia coli BL21 using H3K4Me2 peptide as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins by fluorescence based assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Lysine-specific histone demethylase 1A (CHEMBL6136) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Drug discovery of histone lysine demethylases (KDMs) inhibitors (progress from 2018 to present).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 6.59 | 6.85 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1197637 | — | — | 1 | 6.85 |
| CHEMBL400345 | COLUMBAMINE | — | 1 | 6.33 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1197637 | — | IC50 | = | 140.0 | nM | 6.85 |
| CHEMBL400345 | COLUMBAMINE | IC50 | = | 470.0 | nM | 6.33 |