Assay Detail
Binding
CHEMBL5133030
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of MIF tautomerase (unknown origin) using 4-HPP as substrate preincubated for 1 hr followed by substrate addition
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Macrophage migration inhibitory factor (CHEMBL2085) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Repurposing old drugs as novel inhibitors of human MIF from structural and functional analysis.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 4.10 | 4.18 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL210858 | — | — | 1 | 4.18 |
| CHEMBL90 | HISTAMINE | 4.0 | 1 | 4.13 |
| CHEMBL3526436 | — | — | 1 | 4.07 |
| CHEMBL1201319 | METARAMINOL | 4.0 | 1 | 4.01 |
| CHEMBL165790 | IPRIFLAVONE | 2.0 | 1 | - |
| CHEMBL64 | ISONIAZID | 4.0 | 1 | - |
| CHEMBL39 | SEROTONIN | 3.0 | 1 | - |
| CHEMBL482581 | ESCULIN | — | 1 | - |
| CHEMBL244743 | ESCULETIN | — | 1 | - |
| CHEMBL1235151 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL210858 | — | IC50 | = | 65300.0 | nM | 4.18 |
| CHEMBL90 | HISTAMINE | IC50 | = | 73600.0 | nM | 4.13 |
| CHEMBL3526436 | — | IC50 | = | 85400.0 | nM | 4.07 |
| CHEMBL1201319 | METARAMINOL | IC50 | = | 98100.0 | nM | 4.01 |
| CHEMBL165790 | IPRIFLAVONE | IC50 | = | 255000.0 | nM | - |
| CHEMBL64 | ISONIAZID | IC50 | > | 400000.0 | nM | - |
| CHEMBL39 | SEROTONIN | IC50 | > | 400000.0 | nM | - |
| CHEMBL482581 | ESCULIN | IC50 | = | 160000.0 | nM | - |
| CHEMBL244743 | ESCULETIN | IC50 | = | 211000.0 | nM | - |
| CHEMBL1235151 | — | IC50 | > | 400000.0 | nM | - |