Assay Detail
Binding
CHEMBL5133034
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of MIF tautomerase (unknown origin) using 4-HPP as substrate preincubated for 1 hr followed by substrate addition by Lineweaver-Burk plot anlysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Macrophage migration inhibitory factor (CHEMBL2085) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Repurposing old drugs as novel inhibitors of human MIF from structural and functional analysis.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 6 | 4.22 | 4.36 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL210858 | — | — | 1 | 4.36 |
| CHEMBL90 | HISTAMINE | 4.0 | 1 | 4.21 |
| CHEMBL3526436 | — | — | 1 | 4.21 |
| CHEMBL1201319 | METARAMINOL | 4.0 | 1 | 4.11 |
| CHEMBL482581 | ESCULIN | — | 1 | - |
| CHEMBL244743 | ESCULETIN | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL210858 | — | Ki | = | 43500.0 | nM | 4.36 |
| CHEMBL90 | HISTAMINE | Ki | = | 62000.0 | nM | 4.21 |
| CHEMBL3526436 | — | Ki | = | 61800.0 | nM | 4.21 |
| CHEMBL1201319 | METARAMINOL | Ki | = | 77100.0 | nM | 4.11 |
| CHEMBL482581 | ESCULIN | Ki | = | 143000.0 | nM | - |
| CHEMBL244743 | ESCULETIN | Ki | = | 184000.0 | nM | - |