Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5136137

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human mineralocorticoid receptor S810L mutant expressed in CHO-K1 cells assessed as inhibition of aldosterone-induced transcriptional activity incubated for 24 hrs in presence of 0.5 nM aldosterone by Steady-Glo reagent based reporter gene assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of Apararenone (MT-3995) as a Highly Selective, Potent, and Novel Nonsteroidal Mineralocorticoid Receptor Antagonist.
Iijima T, Katoh M, Takedomi K, Yamamoto Y, Akatsuka H, Shirata N, Nishi A, Takakuwa M, Watanabe Y, Munakata H, Koyama N, Ikeda T, Iguchi T, Kato H, Kikkawa K, Kawaguchi T.
J Med Chem (2022) 65 : 8127 -8143
PubMed 35652647 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.77 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1095097 EPLERENONE 4.0 1 7.00
CHEMBL2181929 MT-3995 2.0 1 6.54

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1095097 EPLERENONE IC50 = 101.0 nM 7.00
CHEMBL2181929 MT-3995 IC50 = 288.0 nM 6.54