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Assay Detail

CHEMBL5136402

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to NLuc human A3 receptor expressed in HEK293 cells assessed as dissociation constant by NanoBRET assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A3 (CHEMBL256)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a High Affinity Adenosine A<sub>1</sub>/A<sub>3</sub> Receptor Antagonist with a Novel 7-Amino-pyrazolo[3,4-<i>d</i>]pyridazine Scaffold.
Suchankova A, Stampelou M, Koutsouki K, Pousias A, Dhingra L, Barkan K, Pouli N, Marakos P, Tenta R, Kolocouris A, Lougiakis N, Ladds G.
ACS Med Chem Lett (2022) 13 : 923 -934
PubMed 35707146 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 1 7.26 7.26

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5195631 1 7.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5195631 Kd = 54.95 nM 7.26