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Assay Detail

CHEMBL5136664

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of His-tagged CDK2/cyclin E1 (unknown origin) expressed in Sf9 cells using histone H1 as substrate in presence of ATP and [gamma33-P]ATP by radioisotope filter binding assay
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Cyclin-dependent kinase 2/cyclin E1 (CHEMBL1907605)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

3,5,7-Substituted Pyrazolo[4,3-<i>d</i>]Pyrimidine Inhibitors of Cyclin-Dependent Kinases and Cyclin K Degraders.
Jorda R, Havlíček L, Peřina M, Vojáčková V, Pospíšil T, Djukic S, Škerlová J, Grúz J, Renešová N, Klener P, Řezáčová P, Strnad M, Kryštof V.
J Med Chem (2022) 65 : 8881 -8896
PubMed 35749742 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 7.75 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5171387 1 8.70
CHEMBL2103840 DINACICLIB 3.0 1 8.52
CHEMBL5182767 1 8.40
CHEMBL5187378 1 8.40
CHEMBL5194202 1 8.40
CHEMBL5209068 1 8.30
CHEMBL5169449 1 8.30
CHEMBL5184795 1 8.30
CHEMBL5207899 1 8.30
CHEMBL5191136 1 8.22
CHEMBL5187158 1 8.22
CHEMBL5183669 1 8.22
CHEMBL5169627 1 8.10
CHEMBL5195127 1 8.05
CHEMBL5176463 1 7.96
CHEMBL5194833 1 7.92
CHEMBL5179519 1 7.92
CHEMBL14762 SELICICLIB 2.0 1 7.75
CHEMBL5205821 1 7.72
CHEMBL5208001 1 7.62
CHEMBL5173574 1 7.60
CHEMBL5176720 1 7.43
CHEMBL5201329 1 7.37
CHEMBL5187017 1 7.28
CHEMBL5201069 1 7.12
CHEMBL5171541 1 6.59
CHEMBL5198145 1 6.33
CHEMBL5199692 1 6.01
CHEMBL5181131 1 5.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5171387 IC50 = 2.0 nM 8.70
CHEMBL2103840 DINACICLIB IC50 = 3.0 nM 8.52
CHEMBL5182767 IC50 = 4.0 nM 8.40
CHEMBL5187378 IC50 = 4.0 nM 8.40
CHEMBL5194202 IC50 = 4.0 nM 8.40
CHEMBL5209068 IC50 = 5.0 nM 8.30
CHEMBL5169449 IC50 = 5.0 nM 8.30
CHEMBL5184795 IC50 = 5.0 nM 8.30
CHEMBL5207899 IC50 = 5.0 nM 8.30
CHEMBL5191136 IC50 = 6.0 nM 8.22
CHEMBL5187158 IC50 = 6.0 nM 8.22
CHEMBL5183669 IC50 = 6.0 nM 8.22
CHEMBL5169627 IC50 = 8.0 nM 8.10
CHEMBL5195127 IC50 = 9.0 nM 8.05
CHEMBL5176463 IC50 = 11.0 nM 7.96
CHEMBL5194833 IC50 = 12.0 nM 7.92
CHEMBL5179519 IC50 = 12.0 nM 7.92
CHEMBL14762 SELICICLIB IC50 = 18.0 nM 7.75
CHEMBL5205821 IC50 = 19.0 nM 7.72
CHEMBL5208001 IC50 = 24.0 nM 7.62
CHEMBL5173574 IC50 = 25.0 nM 7.60
CHEMBL5176720 IC50 = 37.0 nM 7.43
CHEMBL5201329 IC50 = 43.0 nM 7.37
CHEMBL5187017 IC50 = 53.0 nM 7.28
CHEMBL5201069 IC50 = 75.0 nM 7.12
CHEMBL5171541 IC50 = 256.0 nM 6.59
CHEMBL5198145 IC50 = 464.0 nM 6.33
CHEMBL5199692 IC50 = 978.0 nM 6.01
CHEMBL5181131 IC50 = 2302.0 nM 5.64