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Assay Detail

CHEMBL5136722

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Positive allosteric modulation of recombinant human GluN1/GluN2B receptor stably expressed in HEK293 cells assessed as increase in glycine/L-glutamate-induced channel current at -70 mV holding potential by whole cell patch clamp method
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Glutamate NMDA receptor; GRIN1/GRIN2B (CHEMBL1907603)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

SAGE-718: A First-in-Class <i>N</i>-Methyl-d-Aspartate Receptor Positive Allosteric Modulator for the Potential Treatment of Cognitive Impairment.
Hill MD, Blanco MJ, Salituro FG, Bai Z, Beckley JT, Ackley MA, Dai J, Doherty JJ, Harrison BL, Hoffmann EC, Kazdoba TM, Lanzetta D, Lewis M, Quirk MC, Robichaud AJ.
J Med Chem (2022) 65 : 9063 -9075
PubMed 35785990 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 20 6.79 7.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5197305 1 7.64
CHEMBL4448829 1 7.42
CHEMBL5189622 1 7.31
CHEMBL5200422 1 7.22
CHEMBL5095095 DALZANEMDOR 3.0 1 7.10
CHEMBL5198675 1 7.09
CHEMBL5173240 1 7.00
CHEMBL5204245 1 6.96
CHEMBL5198776 1 6.75
CHEMBL5196694 1 6.72
CHEMBL5197411 1 6.60
CHEMBL5172269 1 6.52
CHEMBL5170656 1 6.35
CHEMBL5178963 1 6.23
CHEMBL5170022 1 5.96
CHEMBL5178281 1 5.82
CHEMBL5170297 1 -
CHEMBL5174404 1 -
CHEMBL5175389 1 -
CHEMBL5181802 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5197305 EC50 = 23.0 nM 7.64
CHEMBL4448829 EC50 = 38.0 nM 7.42
CHEMBL5189622 EC50 = 49.0 nM 7.31
CHEMBL5200422 EC50 = 60.0 nM 7.22
CHEMBL5095095 DALZANEMDOR EC50 = 79.0 nM 7.10
CHEMBL5198675 EC50 = 81.0 nM 7.09
CHEMBL5173240 EC50 = 100.0 nM 7.00
CHEMBL5204245 EC50 = 110.0 nM 6.96
CHEMBL5198776 EC50 = 180.0 nM 6.75
CHEMBL5196694 EC50 = 190.0 nM 6.72
CHEMBL5197411 EC50 = 250.0 nM 6.60
CHEMBL5172269 EC50 = 300.0 nM 6.52
CHEMBL5170656 EC50 = 450.0 nM 6.35
CHEMBL5178963 EC50 = 590.0 nM 6.23
CHEMBL5170022 EC50 = 1100.0 nM 5.96
CHEMBL5178281 EC50 = 1500.0 nM 5.82
CHEMBL5170297 EC50 > 10000.0 nM -
CHEMBL5174404 EC50 > 10000.0 nM -
CHEMBL5175389 EC50 > 10000.0 nM -
CHEMBL5181802 EC50 > 10000.0 nM -