Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5136852

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ROCK in rat A7R5 cells assessed as cofilin serine 3 phosphorylation incubated for 1 hr by ICW assay
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type A7R5
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Identification of a Potent, Selective, and Brain-Penetrant Rho Kinase Inhibitor and its Activity in a Mouse Model of Huntington's Disease.
Ladduwahetty T, Lee MR, Maillard MC, Cachope R, Todd D, Barnes M, Beaumont V, Chauhan A, Gallati C, Haughan AF, Kempf G, Luckhurst CA, Matthews K, McAllister G, Mitchell P, Patel H, Rose M, Saville-Stones E, Steinbacher S, Stott AJ, Thatcher E, Tierney J, Urbonas L, Munoz-Sanjuan I, Dominguez C.
J Med Chem (2022) 65 : 9819 -9845
PubMed 35816678 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 6.63 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5206259 1 8.10
CHEMBL4448806 1 8.05
CHEMBL5192610 1 7.85
CHEMBL5208605 1 7.82
CHEMBL5186165 1 7.75
CHEMBL5199904 1 7.48
CHEMBL5181094 1 7.36
CHEMBL5200987 1 7.15
CHEMBL3580669 1 7.13
CHEMBL4459800 1 7.11
CHEMBL5169406 1 6.97
CHEMBL5188032 1 6.91
CHEMBL5183751 1 6.90
CHEMBL5180112 1 6.79
CHEMBL5209364 1 6.60
CHEMBL5174891 1 6.58
CHEMBL5176687 1 6.54
CHEMBL5201650 1 6.49
CHEMBL5171140 1 6.17
CHEMBL5187325 1 6.14
CHEMBL3581146 1 6.08
CHEMBL5193152 1 6.02
CHEMBL5184099 1 6.01
CHEMBL5207169 1 5.99
CHEMBL5209466 1 5.84
CHEMBL559147 Y-27632 1 5.52
CHEMBL5171976 1 5.48
CHEMBL1233300 HYDROXYFASUDIL 1 5.42
CHEMBL5186844 1 5.37
CHEMBL5205297 1 5.31

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5206259 IC50 = 8.0 nM 8.10
CHEMBL4448806 IC50 = 9.0 nM 8.05
CHEMBL5192610 IC50 = 14.0 nM 7.85
CHEMBL5208605 IC50 = 15.0 nM 7.82
CHEMBL5186165 IC50 = 18.0 nM 7.75
CHEMBL5199904 IC50 = 33.0 nM 7.48
CHEMBL5181094 IC50 = 44.0 nM 7.36
CHEMBL5200987 IC50 = 71.0 nM 7.15
CHEMBL3580669 IC50 = 74.0 nM 7.13
CHEMBL4459800 IC50 = 77.0 nM 7.11
CHEMBL5169406 IC50 = 108.0 nM 6.97
CHEMBL5188032 IC50 = 122.0 nM 6.91
CHEMBL5183751 IC50 = 126.0 nM 6.90
CHEMBL5180112 IC50 = 164.0 nM 6.79
CHEMBL5209364 IC50 = 249.0 nM 6.60
CHEMBL5174891 IC50 = 264.0 nM 6.58
CHEMBL5176687 IC50 = 290.0 nM 6.54
CHEMBL5201650 IC50 = 323.0 nM 6.49
CHEMBL5171140 IC50 = 673.0 nM 6.17
CHEMBL5187325 IC50 = 721.0 nM 6.14
CHEMBL3581146 IC50 = 830.0 nM 6.08
CHEMBL5193152 IC50 = 963.0 nM 6.02
CHEMBL5184099 IC50 = 974.0 nM 6.01
CHEMBL5207169 IC50 = 1030.0 nM 5.99
CHEMBL5209466 IC50 = 1460.0 nM 5.84
CHEMBL559147 Y-27632 IC50 = 3026.0 nM 5.52
CHEMBL5171976 IC50 = 3310.0 nM 5.48
CHEMBL1233300 HYDROXYFASUDIL IC50 = 3820.0 nM 5.42
CHEMBL5186844 IC50 = 4270.0 nM 5.37
CHEMBL5205297 IC50 = 4930.0 nM 5.31
CHEMBL5197969 IC50 > 5000.0 nM -
CHEMBL5187811 IC50 > 5000.0 nM -
CHEMBL5207486 IC50 > 5000.0 nM -