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Assay Detail

CHEMBL5138538

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant full-length DNMT1 expressed in Sf9 cells assessed as inhibition of methylated dI-dC formation using poly dI-dC and [Me-3H]SAM as substrate incubated for 2 hrs by liquid scintillation counting analysis
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

DNA (cytosine-5)-methyltransferase 1 (CHEMBL1993)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and Characterization of Transition-State Analogue Inhibitors against Human DNA Methyltransferase 1.
Lamiable-Oulaidi F, Harijan RK, Shaffer KJ, Crump DR, Sun Y, Du Q, Gulab SA, Khan AA, Luxenburger A, Woolhouse AD, Sidoli S, Tyler PC, Schramm VL.
J Med Chem (2022) 65 : 5462 -5494
PubMed 35324190 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 5.54 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5187655 1 6.40
CHEMBL4170114 1 6.40
CHEMBL418052 S-ADENOSYLHOMOCYSTEINE 1 6.16
CHEMBL5189142 1 6.05
CHEMBL5178532 1 5.96
CHEMBL5191050 1 5.92
CHEMBL5188258 1 5.57
CHEMBL5193031 1 5.54
CHEMBL5187336 1 5.44
CHEMBL5173333 1 5.14
CHEMBL5192832 1 5.07
CHEMBL5200086 1 4.99
CHEMBL5174871 1 4.99
CHEMBL5207209 1 4.76
CHEMBL5188584 1 4.71
CHEMBL5189315 1 -
CHEMBL5170757 1 -
CHEMBL5172874 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5187655 IC50 = 400.0 nM 6.40
CHEMBL4170114 IC50 = 400.0 nM 6.40
CHEMBL418052 S-ADENOSYLHOMOCYSTEINE IC50 = 700.0 nM 6.16
CHEMBL5189142 IC50 = 900.0 nM 6.05
CHEMBL5178532 IC50 = 1100.0 nM 5.96
CHEMBL5191050 IC50 = 1200.0 nM 5.92
CHEMBL5188258 IC50 = 2700.0 nM 5.57
CHEMBL5193031 IC50 = 2900.0 nM 5.54
CHEMBL5187336 IC50 = 3600.0 nM 5.44
CHEMBL5173333 IC50 = 7200.0 nM 5.14
CHEMBL5192832 IC50 = 8500.0 nM 5.07
CHEMBL5200086 IC50 = 10300.0 nM 4.99
CHEMBL5174871 IC50 = 10300.0 nM 4.99
CHEMBL5207209 IC50 = 17200.0 nM 4.76
CHEMBL5188584 IC50 = 19600.0 nM 4.71
CHEMBL5189315 IC50 > 100000.0 nM -
CHEMBL5170757 IC50 > 100000.0 nM -
CHEMBL5172874 IC50 > 100000.0 nM -