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Assay Detail

CHEMBL5138944

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human full length recombinant GST-tagged PRMT1 (2 to 371 residues) using histone H4 as substrate incubated for 20 mins in presence of [3H]-SAM by radioisotope-based filter assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein arginine N-methyltransferase 1 (CHEMBL5524)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Turning Nonselective Inhibitors of Type I Protein Arginine Methyltransferases into Potent and Selective Inhibitors of Protein Arginine Methyltransferase 4 through a Deconstruction-Reconstruction and Fragment-Growing Approach.
Iannelli G, Milite C, Marechal N, Cura V, Bonnefond L, Troffer-Charlier N, Feoli A, Rescigno D, Wang Y, Cipriano A, Viviano M, Bedford MT, Cavarelli J, Castellano S, Sbardella G.
J Med Chem (2022) 65 : 11574 -11606
PubMed 35482954 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.41 6.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5173678 1 6.08
CHEMBL5180108 1 6.07
CHEMBL5191769 1 5.75
CHEMBL5186617 1 5.58
CHEMBL5202088 1 5.31
CHEMBL5206272 1 5.07
CHEMBL5170047 1 4.93
CHEMBL5199380 1 4.49

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5173678 IC50 = 835.0 nM 6.08
CHEMBL5180108 IC50 = 860.0 nM 6.07
CHEMBL5191769 IC50 = 1800.0 nM 5.75
CHEMBL5186617 IC50 = 2600.0 nM 5.58
CHEMBL5202088 IC50 = 4860.0 nM 5.31
CHEMBL5206272 IC50 = 8460.0 nM 5.07
CHEMBL5170047 IC50 = 11670.0 nM 4.93
CHEMBL5199380 IC50 = 32270.0 nM 4.49