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Assay Detail

CHEMBL5138982

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human N-terminal GST-tagged mTOR assessed as phosphorylated 4EBP1 by TR-FRET assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase mTOR (CHEMBL2842)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of NVP-CLR457 as an Orally Bioavailable Non-CNS-Penetrant pan-Class IA Phosphoinositol-3-Kinase Inhibitor.
Fairhurst RA, Furet P, Imbach-Weese P, Stauffer F, Rueeger H, McCarthy C, Ripoche S, Oswald S, Arnaud B, Jary A, Maira M, Schnell C, Guthy DA, Wartmann M, Kiffe M, Desrayaud S, Blasco F, Widmer T, Seiler F, Gutmann S, Knapp M, Caravatti G.
J Med Chem (2022) 65 : 8345 -8379
PubMed 35500094 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.15 8.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1879463 DACTOLISIB 3.0 1 8.07
CHEMBL2017976 1 6.36
CHEMBL2017974 BUPARLISIB 3.0 1 5.65
CHEMBL521851 PICTILISIB 2.0 1 5.47
CHEMBL2017977 1 5.20
CHEMBL5192813 1 -
CHEMBL5173332 1 -
CHEMBL5173182 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1879463 DACTOLISIB IC50 = 8.6 nM 8.07
CHEMBL2017976 IC50 = 440.0 nM 6.36
CHEMBL2017974 BUPARLISIB IC50 = 2259.0 nM 5.65
CHEMBL521851 PICTILISIB IC50 = 3351.0 nM 5.47
CHEMBL2017977 IC50 = 6309.0 nM 5.20
CHEMBL5192813 IC50 > 9100.0 nM -
CHEMBL5173332 IC50 > 9100.0 nM -
CHEMBL5173182 IC50 > 9100.0 nM -