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Assay Detail

CHEMBL5144253

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Functional
Leishmanicidal activity against GFP-expressing axenic amastigote stage of Leishmania infantum MCAN/ES/89/IPZ229/1/89 infected in human THP-1 macrophage cells assessed as reduction in intracellular amastigotes incubated for 48 hrs by propidium iodide staining based flow cytometry
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Leishmania infantum
Cell Type THP-1
Confidence 1 — Organism
Curated By Autocuration

Target

Leishmania infantum (CHEMBL612848)
Type ORGANISM
Organism Leishmania infantum

Publication

Pyridazino-pyrrolo-quinoxalinium salts as highly potent and selective leishmanicidal agents targeting trypanothione reductase.
de Lucio H, García-Marín J, Sánchez-Alonso P, García-Soriano JC, Toro MÁ, Vaquero JJ, Gago F, Alajarín R, Jiménez-Ruiz A.
Eur J Med Chem (2022) 227 : 113915 -113915
PubMed 34695777 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 5.77 6.26

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL125 MILTEFOSINE 4.0 1 6.26
CHEMBL5170383 1 5.91
CHEMBL5169813 1 5.75
CHEMBL5189111 1 5.51
CHEMBL5196304 1 5.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL125 MILTEFOSINE EC50 = 550.0 nM 6.26
CHEMBL5170383 EC50 = 1230.0 nM 5.91
CHEMBL5169813 EC50 = 1780.0 nM 5.75
CHEMBL5189111 EC50 = 3100.0 nM 5.51
CHEMBL5196304 EC50 = 4020.0 nM 5.40