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Assay Detail

CHEMBL5144467

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to calf thymus DNA assessed as intercalation by measuring dissociation constant at 20 uM measured after 5 mins by UV-Vis spectrophotometric titration analysis
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 3 — Cell-line / Tissue
Curated By Autocuration

Target

Calf thymus DNA (CHEMBL612514)
Type NUCLEIC-ACID
Organism Bos taurus

Publication

Acridine-O<sup>6</sup>-benzylguanine hybrids: Synthesis, DNA binding, MGMT inhibition and antiproliferative activity.
Franco Pinto J, Fillion A, Duchambon P, Bombard S, Granzhan A.
Eur J Med Chem (2022) 227 : 113909 -113909
PubMed 34731767 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 7 4.77 4.87

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5205895 1 4.87
CHEMBL5203243 1 4.84
CHEMBL76954 1 4.79
CHEMBL5200059 1 4.79
CHEMBL5185198 1 4.72
CHEMBL5189291 1 4.63
CHEMBL5207519 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5205895 Kd = 13400.0 nM 4.87
CHEMBL5203243 Kd = 14300.0 nM 4.84
CHEMBL76954 Kd = 16100.0 nM 4.79
CHEMBL5200059 Kd = 16200.0 nM 4.79
CHEMBL5185198 Kd = 19100.0 nM 4.72
CHEMBL5189291 Kd = 23400.0 nM 4.63
CHEMBL5207519 Kd - -