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Assay Detail

CHEMBL5147131

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]epibatidine from human alpha4beta2 nAChR expressed in HEK293 cell membrane incubated for 3 hrs by scinitillation counting analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Subcellular Cell membrane
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907589)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Pyrrolidinyl benzofurans and benzodioxanes: Selective α4β2 nicotinic acetylcholine receptor ligands with different activity profiles at the two receptor stoichiometries.
Appiani R, Pallavicini M, Hamouda AK, Bolchi C.
Bioorg Med Chem Lett (2022) 65 : 128701 -128701
PubMed 35346843 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 6.33 6.76

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5183330 1 6.76
CHEMBL433777 1 6.14
CHEMBL5186760 1 6.08
CHEMBL5200048 1 -
CHEMBL5178221 1 -
CHEMBL5200745 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5183330 Ki = 172.0 nM 6.76
CHEMBL433777 Ki = 718.0 nM 6.14
CHEMBL5186760 Ki = 828.0 nM 6.08
CHEMBL5200048 Ki > 100000.0 nM -
CHEMBL5178221 Ki > 100000.0 nM -
CHEMBL5200745 Ki = 181000.0 nM -