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Assay Detail

CHEMBL5148333

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of wild type N-terminal GST tagged ALK cytoplasmic domain (1058 to 1620 end residues) (unknown origin) expressed in Sf21 cells incubated for 1 hr in presence of ATP
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ALK tyrosine kinase receptor (CHEMBL4247)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 2,4-diarylaminopyrimidine derivatives bearing dithiocarbamate moiety as novel ALK inhibitors.
Wang X, Hu Y, Zou X, Wang P, Yue H, Guo M, Li Z, Gong P.
Bioorg Med Chem (2022) 66 : 116794 -116794
PubMed 35576654 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.95 8.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2403108 CERITINIB 4.0 1 8.92
CHEMBL5179806 1 8.34
CHEMBL5178030 1 8.19
CHEMBL5209011 1 7.96
CHEMBL5199005 1 7.80
CHEMBL5169860 1 7.29
CHEMBL5176307 1 7.12
CHEMBL5175979 1 -
CHEMBL5189267 1 -
CHEMBL5199475 1 -
CHEMBL5179387 1 -
CHEMBL5178846 1 -
CHEMBL5185272 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2403108 CERITINIB IC50 = 1.2 nM 8.92
CHEMBL5179806 IC50 = 4.59 nM 8.34
CHEMBL5178030 IC50 = 6.5 nM 8.19
CHEMBL5209011 IC50 = 11.0 nM 7.96
CHEMBL5199005 IC50 = 16.0 nM 7.80
CHEMBL5169860 IC50 = 51.0 nM 7.29
CHEMBL5176307 IC50 = 75.0 nM 7.12
CHEMBL5175979 IC50 > 100.0 nM -
CHEMBL5189267 IC50 > 100.0 nM -
CHEMBL5199475 IC50 > 100.0 nM -
CHEMBL5179387 IC50 > 100.0 nM -
CHEMBL5178846 IC50 > 100.0 nM -
CHEMBL5185272 IC50 > 100.0 nM -