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Assay Detail

CHEMBL5148669

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length Mycobacterium tuberculosis protein kinase G using His-tagged GarA as substrate in presence of [gamma32P] ATP measured after 12 to 24 hrs by radiometric kinase assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mycobacterium tuberculosis
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase PknG (CHEMBL6070)
Type SINGLE PROTEIN
Organism Mycobacterium tuberculosis

Publication

Cosolvent Sites-Based Discovery of <i>Mycobacterium Tuberculosis</i> Protein Kinase G Inhibitors.
Burastero O, Defelipe LA, Gola G, Tateosian NL, Lopez ED, Martinena CB, Arcon JP, Traian MD, Wetzler DE, Bento I, Barril X, Ramirez J, Marti MA, Garcia-Alai MM, Turjanski AG.
J Med Chem (2022) 65 : 9691 -9705
PubMed 35737472 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 5.65 6.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL379367 1 6.05
CHEMBL5200480 1 5.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL379367 IC50 = 900.0 nM 6.05
CHEMBL5200480 IC50 = 5800.0 nM 5.24