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Assay Detail

CHEMBL5150899

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human PAK4 expressed in TR-293-KDG cells assessed as inhibition of GEFH1 phosphorylation at S180 using GEFH1 as substrate incubated for 3 hrs by ELISA
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type TR-293-KDG
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase PAK 4 (CHEMBL4482)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structural insights of oxindole based kinase inhibitors as anticancer agents: Recent advances.
Dhokne P, Sakla AP, Shankaraiah N.
Eur J Med Chem (2021) 216 : 113334 -113334
PubMed 33721669 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 8.89 8.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3128043 PF-03758309 1.0 1 8.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3128043 PF-03758309 IC50 = 1.3 nM 8.89