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Assay Detail

CHEMBL5151019

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glutathione and ATP by HTRF assay
34
Total Activities
34
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

NEDD8 activating enzyme (CHEMBL3831283)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Development of Potent NEDD8-Activating Enzyme Inhibitors Bearing a Pyrimidotriazole Scaffold.
Xiong C, Zhou L, Tan J, Song S, Bao X, Zhang N, Ding H, Zhao J, He JX, Miao ZH, Zhang A.
J Med Chem (2021) 64 : 6161 -6178
PubMed 33857374 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 34 7.96 10.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5170838 1 10.70
CHEMBL5182746 1 9.68
CHEMBL1231160 PEVONEDISTAT 3.0 1 9.59
CHEMBL5177755 1 9.26
CHEMBL5206652 1 9.24
CHEMBL5173723 1 8.65
CHEMBL5192493 1 8.64
CHEMBL5197178 1 8.45
CHEMBL5209054 1 8.38
CHEMBL5188530 1 8.24
CHEMBL5193236 1 8.22
CHEMBL5177700 1 8.21
CHEMBL5178136 1 8.01
CHEMBL5192527 1 8.00
CHEMBL5169743 1 7.80
CHEMBL5170141 1 7.68
CHEMBL5179467 1 7.68
CHEMBL5207989 1 7.51
CHEMBL5201960 1 7.17
CHEMBL5206633 1 7.09
CHEMBL5206263 1 7.09
CHEMBL5190183 1 6.97
CHEMBL5188300 1 6.92
CHEMBL5200375 1 6.83
CHEMBL5173277 1 6.77
CHEMBL5188093 1 6.73
CHEMBL5189807 1 6.68
CHEMBL5197934 1 6.66
CHEMBL5187203 1 -
CHEMBL5197609 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5170838 IC50 = 0.02 nM 10.70
CHEMBL5182746 IC50 = 0.21 nM 9.68
CHEMBL1231160 PEVONEDISTAT IC50 = 0.26 nM 9.59
CHEMBL5177755 IC50 = 0.55 nM 9.26
CHEMBL5206652 IC50 = 0.57 nM 9.24
CHEMBL5173723 IC50 = 2.23 nM 8.65
CHEMBL5192493 IC50 = 2.28 nM 8.64
CHEMBL5197178 IC50 = 3.56 nM 8.45
CHEMBL5209054 IC50 = 4.2 nM 8.38
CHEMBL5188530 IC50 = 5.77 nM 8.24
CHEMBL5193236 IC50 = 6.0 nM 8.22
CHEMBL5177700 IC50 = 6.11 nM 8.21
CHEMBL5178136 IC50 = 9.76 nM 8.01
CHEMBL5192527 IC50 = 9.98 nM 8.00
CHEMBL5169743 IC50 = 15.9 nM 7.80
CHEMBL5170141 IC50 = 21.1 nM 7.68
CHEMBL5179467 IC50 = 21.1 nM 7.68
CHEMBL5207989 IC50 = 30.6 nM 7.51
CHEMBL5201960 IC50 = 66.9 nM 7.17
CHEMBL5206633 IC50 = 81.8 nM 7.09
CHEMBL5206263 IC50 = 80.9 nM 7.09
CHEMBL5190183 IC50 = 107.0 nM 6.97
CHEMBL5188300 IC50 = 120.0 nM 6.92
CHEMBL5200375 IC50 = 149.0 nM 6.83
CHEMBL5173277 IC50 = 170.0 nM 6.77
CHEMBL5188093 IC50 = 187.0 nM 6.73
CHEMBL5189807 IC50 = 207.0 nM 6.68
CHEMBL5197934 IC50 = 219.0 nM 6.66
CHEMBL5187203 IC50 > 10000.0 nM -
CHEMBL5197609 IC50 > 10000.0 nM -
CHEMBL5183530 IC50 > 10000.0 nM -
CHEMBL5173798 IC50 > 10000.0 nM -
CHEMBL5199010 IC50 > 10000.0 nM -
CHEMBL5201980 IC50 > 10000.0 nM -