Assay Detail
Binding
CHEMBL5151486
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of [3H]N-methylspiperone from human D4.4 receptor stably expressed in HEK293T cells co-expressing ElucN-betaarr2 hD4.4R-ELuc incubated for 60 mins by scintillation counting analysis
8
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293T |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Abolishing Dopamine D<sub>2long</sub>/D<sub>3</sub> Receptor Affinity of Subtype-Selective Carbamoylguanidine-Type Histamine H<sub>2</sub> Receptor Agonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 4 | 10.11 | 10.11 |
| EC50 | 4 | 5.99 | 6.96 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4860528 | — | — | 2 | 10.11 |
| CHEMBL5207281 | — | — | 2 | 10.11 |
| CHEMBL5206565 | — | — | 2 | 10.11 |
| CHEMBL5201074 | — | — | 2 | 10.11 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4860528 | — | Kd | = | 0.078 | nM | 10.11 |
| CHEMBL5207281 | — | Kd | = | 0.078 | nM | 10.11 |
| CHEMBL5206565 | — | Kd | = | 0.078 | nM | 10.11 |
| CHEMBL5201074 | — | Kd | = | 0.078 | nM | 10.11 |
| CHEMBL4860528 | — | EC50 | = | 109.65 | nM | 6.96 |
| CHEMBL5206565 | — | EC50 | = | 676.08 | nM | 6.17 |
| CHEMBL5207281 | — | EC50 | = | 2238.72 | nM | 5.65 |
| CHEMBL5201074 | — | EC50 | = | 6606.93 | nM | 5.18 |