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Assay Detail

CHEMBL5151608

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of porcine brain GSK-3alpha/beta incubated for 30 mins in presence of [33p]-gamma ATP by scintillation counter analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Glycogen synthase kinase-3 (CHEMBL2366565)
Type PROTEIN FAMILY
Organism Sus scrofa

Publication

Anticancer potential of indirubins in medicinal chemistry: Biological activity, structural modification, and structure-activity relationship.
Wang H, Wang Z, Wei C, Wang J, Xu Y, Bai G, Yao Q, Zhang L, Chen Y.
Eur J Med Chem (2021) 223 : 113652 -113652
PubMed 34161865 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.62 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL409450 6BIO 1 8.30
CHEMBL216543 1 7.66
CHEMBL1276127 INDIRUBIN 2.0 1 6.00
CHEMBL1092509 1 4.50
CHEMBL5199235 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL409450 6BIO IC50 = 5.0 nM 8.30
CHEMBL216543 IC50 = 22.0 nM 7.66
CHEMBL1276127 INDIRUBIN IC50 = 1000.0 nM 6.00
CHEMBL1092509 IC50 = 32000.0 nM 4.50
CHEMBL5199235 IC50 > 100000.0 nM -