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Assay Detail

CHEMBL5152029

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant N-terminal Avi-6His-TEV tagged PARP2 full length expressed in pFastBac expression system incubated for 4 hrs by fluorescence anisotropy binding assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Poly [ADP-ribose] polymerase 2 (CHEMBL5366)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 5-{4-[(7-Ethyl-6-oxo-5,6-dihydro-1,5-naphthyridin-3-yl)methyl]piperazin-1-yl}-<i>N</i>-methylpyridine-2-carboxamide (AZD5305): A PARP1-DNA Trapper with High Selectivity for PARP1 over PARP2 and Other PARPs.
Johannes JW, Balazs A, Barratt D, Bista M, Chuba MD, Cosulich S, Critchlow SE, Degorce SL, Di Fruscia P, Edmondson SD, Embrey K, Fawell S, Ghosh A, Gill SJ, Gunnarsson A, Hande SM, Heightman TD, Hemsley P, Illuzzi G, Lane J, Larner C, Leo E, Liu L, Madin A, Martin S, McWilliams L, O'Connor MJ, Orme JP, Pachl F, Packer MJ, Pei X, Pike A, Schimpl M, She H, Staniszewska AD, Talbot V, Underwood E, Varnes JG, Xue L, Yao T, Zhang K, Zhang AX, Zheng X.
J Med Chem (2021) 64 : 14498 -14512
PubMed 34570508 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 5.74 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL521686 OLAPARIB 4.0 1 8.22
CHEMBL3137320 TALAZOPARIB 4.0 1 7.52
CHEMBL1173055 RUCAPARIB 4.0 1 6.76
CHEMBL506871 VELIPARIB 3.0 1 6.65
CHEMBL5177596 1 6.31
CHEMBL5191396 1 6.22
CHEMBL5197101 1 5.96
CHEMBL5209181 1 5.96
CHEMBL5095220 SARUPARIB 3.0 1 5.85
CHEMBL4874589 1 5.77
CHEMBL1094636 NIRAPARIB 4.0 1 5.75
CHEMBL5183769 1 5.62
CHEMBL5200723 1 5.41
CHEMBL5193246 1 5.40
CHEMBL5181905 1 5.38
CHEMBL5188853 1 5.31
CHEMBL5195580 1 5.23
CHEMBL3606021 1 5.18
CHEMBL5178594 1 5.18
CHEMBL5202060 1 5.13
CHEMBL5197824 1 5.04
CHEMBL251030 1 4.70
CHEMBL5190481 1 4.66
CHEMBL5176615 1 4.55
CHEMBL5196187 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL521686 OLAPARIB IC50 = 6.0 nM 8.22
CHEMBL3137320 TALAZOPARIB IC50 = 30.0 nM 7.52
CHEMBL1173055 RUCAPARIB IC50 = 172.0 nM 6.76
CHEMBL506871 VELIPARIB IC50 = 225.0 nM 6.65
CHEMBL5177596 IC50 = 490.0 nM 6.31
CHEMBL5191396 IC50 = 600.0 nM 6.22
CHEMBL5197101 IC50 = 1100.0 nM 5.96
CHEMBL5209181 IC50 = 1100.0 nM 5.96
CHEMBL5095220 SARUPARIB IC50 = 1400.0 nM 5.85
CHEMBL4874589 IC50 = 1700.0 nM 5.77
CHEMBL1094636 NIRAPARIB IC50 = 1800.0 nM 5.75
CHEMBL5183769 IC50 = 2400.0 nM 5.62
CHEMBL5200723 IC50 = 3900.0 nM 5.41
CHEMBL5193246 IC50 = 4000.0 nM 5.40
CHEMBL5181905 IC50 = 4200.0 nM 5.38
CHEMBL5188853 IC50 = 4900.0 nM 5.31
CHEMBL5195580 IC50 = 5900.0 nM 5.23
CHEMBL3606021 IC50 = 6600.0 nM 5.18
CHEMBL5178594 IC50 = 6600.0 nM 5.18
CHEMBL5202060 IC50 = 7400.0 nM 5.13
CHEMBL5197824 IC50 = 9100.0 nM 5.04
CHEMBL251030 IC50 = 20000.0 nM 4.70
CHEMBL5190481 IC50 = 22000.0 nM 4.66
CHEMBL5176615 IC50 = 28000.0 nM 4.55
CHEMBL5196187 IC50 > 26000.0 nM -