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Assay Detail

CHEMBL5153773

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human PDE5 using [3H]cGMP as substrate incubated for 10 mins by liquid scintillation counter analysis
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

cGMP-specific 3',5'-cyclic phosphodiesterase (CHEMBL1827)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Computational design, synthesis and biological evaluation of PDE5 inhibitors based on N<sup>2</sup>,N<sup>4</sup>-diaminoquinazoline and N<sup>2</sup>,N<sup>6</sup>-diaminopurine scaffolds.
Somnarin T, Pobsuk N, Chantakul R, Panklai T, Temkitthawon P, Hannongbua S, Chootip K, Ingkaninan K, Boonyarattanakalin K, Gleeson D, Paul Gleeson M.
Bioorg Med Chem (2022) 76 : 117092 -117092
PubMed 36450167 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 5.80 8.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL192 SILDENAFIL 4.0 1 8.85
CHEMBL4445920 1 7.00
CHEMBL5175706 1 6.82
CHEMBL5178863 1 6.66
CHEMBL5194921 1 6.54
CHEMBL5187496 1 6.44
CHEMBL5194783 1 6.39
CHEMBL5184989 1 6.37
CHEMBL5203939 1 6.31
CHEMBL5186856 1 6.30
CHEMBL5195499 1 6.28
CHEMBL5191104 1 6.22
CHEMBL5200202 1 5.99
CHEMBL5199387 1 5.84
CHEMBL5188731 1 5.79
CHEMBL5177893 1 5.70
CHEMBL5195434 1 5.47
CHEMBL5171693 1 5.40
CHEMBL5183230 1 5.35
CHEMBL5208363 1 5.28
CHEMBL5193660 1 5.28
CHEMBL5196307 1 5.03
CHEMBL5183757 1 4.72
CHEMBL5206939 1 4.64
CHEMBL5195857 1 4.54
CHEMBL5193806 1 4.48
CHEMBL5188011 1 4.42
CHEMBL5200266 1 4.37
CHEMBL5170729 1 -
CHEMBL5191329 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL192 SILDENAFIL IC50 = 1.4 nM 8.85
CHEMBL4445920 IC50 = 100.0 nM 7.00
CHEMBL5175706 IC50 = 150.0 nM 6.82
CHEMBL5178863 IC50 = 220.0 nM 6.66
CHEMBL5194921 IC50 = 290.0 nM 6.54
CHEMBL5187496 IC50 = 360.0 nM 6.44
CHEMBL5194783 IC50 = 410.0 nM 6.39
CHEMBL5184989 IC50 = 430.0 nM 6.37
CHEMBL5203939 IC50 = 490.0 nM 6.31
CHEMBL5186856 IC50 = 500.0 nM 6.30
CHEMBL5195499 IC50 = 520.0 nM 6.28
CHEMBL5191104 IC50 = 600.0 nM 6.22
CHEMBL5200202 IC50 = 1030.0 nM 5.99
CHEMBL5199387 IC50 = 1440.0 nM 5.84
CHEMBL5188731 IC50 = 1640.0 nM 5.79
CHEMBL5177893 IC50 = 2020.0 nM 5.70
CHEMBL5195434 IC50 = 3410.0 nM 5.47
CHEMBL5171693 IC50 = 3970.0 nM 5.40
CHEMBL5183230 IC50 = 4460.0 nM 5.35
CHEMBL5208363 IC50 = 5230.0 nM 5.28
CHEMBL5193660 IC50 = 5280.0 nM 5.28
CHEMBL5196307 IC50 = 9390.0 nM 5.03
CHEMBL5183757 IC50 = 18880.0 nM 4.72
CHEMBL5206939 IC50 = 23010.0 nM 4.64
CHEMBL5195857 IC50 = 28540.0 nM 4.54
CHEMBL5193806 IC50 = 33260.0 nM 4.48
CHEMBL5188011 IC50 = 38370.0 nM 4.42
CHEMBL5200266 IC50 = 43160.0 nM 4.37
CHEMBL5170729 IC50 - -
CHEMBL5191329 IC50 - -
CHEMBL5180347 IC50 - -