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Assay Detail

CHEMBL5155268

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PI3Kgamma in SDF-1alpha stimulated human whole blood assessed as reduction in phospho-AKT kinase activity preincubated for 1 hr followed by SDF-1alpha stimulation and measured after 2 mins by flow cytometric analysis
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Blood
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Design, Synthesis, and Structure-Activity Relationship Optimization of Pyrazolopyrimidine Amide Inhibitors of Phosphoinositide 3-Kinase γ (PI3Kγ).
Mata G, Miles DH, Drew SL, Fournier J, Lawson KV, Mailyan AK, Sharif EU, Yan X, Beatty JW, Banuelos J, Chen J, Ginn E, Chen A, Gerrick KY, Pham AT, Wong K, Soni D, Dhanota P, Shaqfeh SG, Meleza C, Narasappa N, Singh H, Zhao X, Jin L, Schindler U, Walters MJ, Young SW, Walker NP, Leleti MR, Powers JP, Jeffrey JL.
J Med Chem (2022) 65 : 1418 -1444
PubMed 34672584 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.29 6.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5172719 1 6.68
CHEMBL5196935 1 6.62
CHEMBL5171421 1 6.58
CHEMBL5209268 1 6.52
CHEMBL5206549 1 6.38
CHEMBL5203289 1 6.29
CHEMBL5176293 1 6.22
CHEMBL5201929 1 6.22
CHEMBL5205997 1 6.12
CHEMBL5186453 1 6.10
CHEMBL5189490 1 5.89
CHEMBL5177397 1 5.89
CHEMBL5171703 1 -
CHEMBL5179088 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5172719 IC50 = 210.0 nM 6.68
CHEMBL5196935 IC50 = 240.0 nM 6.62
CHEMBL5171421 IC50 = 260.0 nM 6.58
CHEMBL5209268 IC50 = 300.0 nM 6.52
CHEMBL5206549 IC50 = 420.0 nM 6.38
CHEMBL5203289 IC50 = 510.0 nM 6.29
CHEMBL5176293 IC50 = 600.0 nM 6.22
CHEMBL5201929 IC50 = 600.0 nM 6.22
CHEMBL5205997 IC50 = 750.0 nM 6.12
CHEMBL5186453 IC50 = 800.0 nM 6.10
CHEMBL5189490 IC50 = 1300.0 nM 5.89
CHEMBL5177397 IC50 = 1300.0 nM 5.89
CHEMBL5171703 IC50 - -
CHEMBL5179088 IC50 - -