Assay Detail
Binding
CHEMBL5155787
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Escherichia coli C-terminal hexahistidine tagged DHFR incubated for 5 mins in presence of NADPH by measuring oxidation of NADPH to NADP+
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
The discovery of 1, 3-diamino-7H-pyrrol[3, 2-f]quinazoline compounds as potent antimicrobial antifolates.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 7.66 | 8.63 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5194771 | — | — | 1 | 8.63 |
| CHEMBL5201889 | — | — | 1 | 7.80 |
| CHEMBL22 | TRIMETHOPRIM | 4.0 | 1 | 7.21 |
| CHEMBL525149 | — | — | 1 | 7.01 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5194771 | — | IC50 | = | 2.36 | nM | 8.63 |
| CHEMBL5201889 | — | IC50 | = | 15.93 | nM | 7.80 |
| CHEMBL22 | TRIMETHOPRIM | IC50 | = | 61.6 | nM | 7.21 |
| CHEMBL525149 | — | IC50 | = | 98.36 | nM | 7.01 |