Assay Detail
Binding
CHEMBL5158338
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of full length human recombinant HDAC6 expressed in Sf9 baculovirus system using FAM-labeled acetylated peptide as substrate by measuring fluorescence intensity by EMSA method
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of HDAC6-Selective Inhibitor NN-390 with <i>in Vitro</i> Efficacy in Group 3 Medulloblastoma.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 16 | 7.82 | 8.66 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3693786 | CITARINOSTAT | 2.0 | 1 | 8.66 |
| CHEMBL2364628 | RICOLINOSTAT | 2.0 | 1 | 8.59 |
| CHEMBL5179295 | — | — | 1 | 8.37 |
| CHEMBL98 | VORINOSTAT | 4.0 | 1 | 8.24 |
| CHEMBL5177475 | — | — | 1 | 8.22 |
| CHEMBL5192865 | — | — | 1 | 8.07 |
| CHEMBL2179618 | NEXTURASTAT A | — | 1 | 7.91 |
| CHEMBL4763401 | — | — | 1 | 7.85 |
| CHEMBL5173782 | — | — | 1 | 7.79 |
| CHEMBL5202595 | — | — | 1 | 7.61 |
| CHEMBL5191960 | — | — | 1 | 7.56 |
| CHEMBL5199669 | — | — | 1 | 7.30 |
| CHEMBL5202970 | — | — | 1 | 7.30 |
| CHEMBL5195141 | — | — | 1 | 6.92 |
| CHEMBL5184540 | — | — | 1 | 6.84 |
| CHEMBL5208971 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3693786 | CITARINOSTAT | IC50 | = | 2.21 | nM | 8.66 |
| CHEMBL2364628 | RICOLINOSTAT | IC50 | = | 2.59 | nM | 8.59 |
| CHEMBL5179295 | — | IC50 | = | 4.32 | nM | 8.37 |
| CHEMBL98 | VORINOSTAT | IC50 | = | 5.8 | nM | 8.24 |
| CHEMBL5177475 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL5192865 | — | IC50 | = | 8.5 | nM | 8.07 |
| CHEMBL2179618 | NEXTURASTAT A | IC50 | = | 12.4 | nM | 7.91 |
| CHEMBL4763401 | — | IC50 | = | 14.0 | nM | 7.85 |
| CHEMBL5173782 | — | IC50 | = | 16.2 | nM | 7.79 |
| CHEMBL5202595 | — | IC50 | = | 24.6 | nM | 7.61 |
| CHEMBL5191960 | — | IC50 | = | 27.8 | nM | 7.56 |
| CHEMBL5199669 | — | IC50 | = | 49.9 | nM | 7.30 |
| CHEMBL5202970 | — | IC50 | = | 50.4 | nM | 7.30 |
| CHEMBL5195141 | — | IC50 | = | 121.0 | nM | 6.92 |
| CHEMBL5184540 | — | IC50 | = | 143.0 | nM | 6.84 |
| CHEMBL5208971 | — | IC50 | > | 1000.0 | nM | - |