Assay Detail
Binding
CHEMBL5158356
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Competitive inhibition of N-terminal His-SUMO tagged zebra fish recombinant HDAC6 catalytic domain 2 transfected with pET28b(+) vector in Escherichia coli BL21 (DE3) RILP cells incubated for 10 mins followed by addition of FITC-M344 measured af ter 10 mins by fluorescence polarization assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Danio rerio |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Discovery of HDAC6-Selective Inhibitor NN-390 with <i>in Vitro</i> Efficacy in Group 3 Medulloblastoma.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 6.68 | 7.04 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2364628 | RICOLINOSTAT | 2.0 | 1 | 7.04 |
| CHEMBL5202595 | — | — | 1 | 6.86 |
| CHEMBL5177475 | — | — | 1 | 6.86 |
| CHEMBL3693786 | CITARINOSTAT | 2.0 | 1 | 6.81 |
| CHEMBL5192865 | — | — | 1 | 6.76 |
| CHEMBL5179295 | — | — | 1 | 6.74 |
| CHEMBL408513 | BELINOSTAT | 4.0 | 1 | 6.67 |
| CHEMBL5199669 | — | — | 1 | 6.45 |
| CHEMBL5195141 | — | — | 1 | 5.95 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2364628 | RICOLINOSTAT | IC50 | = | 90.2 | nM | 7.04 |
| CHEMBL5202595 | — | IC50 | = | 137.0 | nM | 6.86 |
| CHEMBL5177475 | — | IC50 | = | 139.0 | nM | 6.86 |
| CHEMBL3693786 | CITARINOSTAT | IC50 | = | 154.0 | nM | 6.81 |
| CHEMBL5192865 | — | IC50 | = | 173.0 | nM | 6.76 |
| CHEMBL5179295 | — | IC50 | = | 182.0 | nM | 6.74 |
| CHEMBL408513 | BELINOSTAT | IC50 | = | 213.0 | nM | 6.67 |
| CHEMBL5199669 | — | IC50 | = | 356.0 | nM | 6.45 |
| CHEMBL5195141 | — | IC50 | = | 1126.0 | nM | 5.95 |