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Assay Detail

CHEMBL5159488

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Trypanosoma cruzi cruzain using Z-FR-AMC as substrate preincubated for 10 mins followed by substrate addition by fluorimetric analysis
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Trypanosoma cruzi
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cruzipain (CHEMBL3563)
Type SINGLE PROTEIN
Organism Trypanosoma cruzi

Publication

Intramolecular Interactions Enhance the Potency of Gallinamide A Analogues against <i>Trypanosoma cruzi</i>.
Barbosa Da Silva E, Sharma V, Hernandez-Alvarez L, Tang AH, Stoye A, O'Donoghue AJ, Gerwick WH, Payne RJ, McKerrow JH, Podust LM.
J Med Chem (2022) 65 : 4255 -4269
PubMed 35188371 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 9.32 10.09

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4596414 1 10.09
CHEMBL4594870 1 10.07
CHEMBL4596647 1 10.05
CHEMBL3558756 1 9.96
CHEMBL5184737 1 9.96
CHEMBL5171874 1 9.92
CHEMBL5175270 1 9.89
CHEMBL4594757 1 9.72
CHEMBL5199542 1 9.59
CHEMBL5176188 1 9.55
CHEMBL4596941 1 9.47
CHEMBL5183942 1 9.40
CHEMBL4595806 1 9.31
CHEMBL5191563 1 9.17
CHEMBL5204190 1 9.16
CHEMBL4597308 1 9.15
CHEMBL4596992 1 9.13
CHEMBL5179302 1 9.10
CHEMBL4595664 1 9.07
CHEMBL4598338 1 8.94
CHEMBL541700 GALLINAMIDE A 1 8.89
CHEMBL4595223 1 8.87
CHEMBL5202965 1 8.81
CHEMBL4595559 1 8.30
CHEMBL5199098 1 7.53

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4596414 IC50 = 0.081 nM 10.09
CHEMBL4594870 IC50 = 0.086 nM 10.07
CHEMBL4596647 IC50 = 0.09 nM 10.05
CHEMBL3558756 IC50 = 0.11 nM 9.96
CHEMBL5184737 IC50 = 0.11 nM 9.96
CHEMBL5171874 IC50 = 0.12 nM 9.92
CHEMBL5175270 IC50 = 0.13 nM 9.89
CHEMBL4594757 IC50 = 0.19 nM 9.72
CHEMBL5199542 IC50 = 0.26 nM 9.59
CHEMBL5176188 IC50 = 0.28 nM 9.55
CHEMBL4596941 IC50 = 0.34 nM 9.47
CHEMBL5183942 IC50 = 0.4 nM 9.40
CHEMBL4595806 IC50 = 0.49 nM 9.31
CHEMBL5191563 IC50 = 0.68 nM 9.17
CHEMBL5204190 IC50 = 0.69 nM 9.16
CHEMBL4597308 IC50 = 0.71 nM 9.15
CHEMBL4596992 IC50 = 0.74 nM 9.13
CHEMBL5179302 IC50 = 0.79 nM 9.10
CHEMBL4595664 IC50 = 0.85 nM 9.07
CHEMBL4598338 IC50 = 1.14 nM 8.94
CHEMBL541700 GALLINAMIDE A IC50 = 1.3 nM 8.89
CHEMBL4595223 IC50 = 1.35 nM 8.87
CHEMBL5202965 IC50 = 1.56 nM 8.81
CHEMBL4595559 IC50 = 4.95 nM 8.30
CHEMBL5199098 IC50 = 29.73 nM 7.53