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Assay Detail

CHEMBL5160914

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against human HCC827 cells expressing EGFR deletion19 mutant assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HCC827
Confidence 1 — Organism
Curated By Autocuration

Target

HCC827 (CHEMBL4296422)
Type CELL-LINE
Organism Homo sapiens

Publication

Discovery of Potent PROTACs Targeting EGFR Mutants through the Optimization of Covalent EGFR Ligands.
Zhao HY, Wang HP, Mao YZ, Zhang H, Xin M, Xi XX, Lei H, Mao S, Li DH, Zhang SQ.
J Med Chem (2022) 65 : 4709 -4726
PubMed 35254067 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 7.90 9.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5185820 1 9.48
CHEMBL5173237 1 9.42
CHEMBL5202214 1 9.33
CHEMBL2178352 1 9.03
CHEMBL3353410 OSIMERTINIB 4.0 1 9.00
CHEMBL5184446 1 8.94
CHEMBL5204635 1 8.80
CHEMBL5208749 1 8.78
CHEMBL5192520 1 8.78
CHEMBL5178536 1 8.75
CHEMBL5179946 1 8.74
CHEMBL5188212 1 8.66
CHEMBL5173023 1 8.61
CHEMBL5179452 1 8.59
CHEMBL5170905 1 8.52
CHEMBL5194346 1 8.27
CHEMBL5178388 1 8.05
CHEMBL5192418 1 7.44
CHEMBL5191971 1 7.22
CHEMBL5184717 1 7.21
CHEMBL5171149 1 7.17
CHEMBL5198335 1 7.12
CHEMBL5174906 1 7.07
CHEMBL5207320 1 6.96
CHEMBL5204884 1 6.89
CHEMBL5199988 1 6.85
CHEMBL5204201 1 6.82
CHEMBL5202759 1 6.26
CHEMBL5187191 1 6.17
CHEMBL5182033 1 6.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5185820 IC50 = 0.33 nM 9.48
CHEMBL5173237 IC50 = 0.38 nM 9.42
CHEMBL5202214 IC50 = 0.47 nM 9.33
CHEMBL2178352 IC50 = 0.93 nM 9.03
CHEMBL3353410 OSIMERTINIB IC50 = 1.0 nM 9.00
CHEMBL5184446 IC50 = 1.16 nM 8.94
CHEMBL5204635 IC50 = 1.6 nM 8.80
CHEMBL5208749 IC50 = 1.67 nM 8.78
CHEMBL5192520 IC50 = 1.66 nM 8.78
CHEMBL5178536 IC50 = 1.77 nM 8.75
CHEMBL5179946 IC50 = 1.83 nM 8.74
CHEMBL5188212 IC50 = 2.19 nM 8.66
CHEMBL5173023 IC50 = 2.48 nM 8.61
CHEMBL5179452 IC50 = 2.55 nM 8.59
CHEMBL5170905 IC50 = 3.05 nM 8.52
CHEMBL5194346 IC50 = 5.39 nM 8.27
CHEMBL5178388 IC50 = 8.93 nM 8.05
CHEMBL5192418 IC50 = 36.5 nM 7.44
CHEMBL5191971 IC50 = 59.61 nM 7.22
CHEMBL5184717 IC50 = 61.0 nM 7.21
CHEMBL5171149 IC50 = 67.0 nM 7.17
CHEMBL5198335 IC50 = 75.0 nM 7.12
CHEMBL5174906 IC50 = 86.0 nM 7.07
CHEMBL5207320 IC50 = 110.0 nM 6.96
CHEMBL5204884 IC50 = 130.0 nM 6.89
CHEMBL5199988 IC50 = 140.0 nM 6.85
CHEMBL5204201 IC50 = 150.0 nM 6.82
CHEMBL5202759 IC50 = 544.0 nM 6.26
CHEMBL5187191 IC50 = 683.0 nM 6.17
CHEMBL5182033 IC50 = 768.0 nM 6.12
CHEMBL5176927 IC50 = 1390.0 nM 5.86
CHEMBL5201463 IC50 > 10000.0 nM -
CHEMBL5181560 IC50 > 10000.0 nM -