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Assay Detail

CHEMBL5161038

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Escherichia coli DHFR preincubated with NADPH for 3 mins followed by compound addition for 7 mins by colorimetric assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Hypothesis-Driven, Structure-Based Design in Photopharmacology: The Case of eDHFR Inhibitors.
Kobauri P, Galenkamp NS, Schulte AM, de Vries J, Simeth NA, Maglia G, Thallmair S, Kolarski D, Szymanski W, Feringa BL.
J Med Chem (2022) 65 : 4798 -4817
PubMed 35258959 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 9.00 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL22 TRIMETHOPRIM 4.0 1 9.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL22 TRIMETHOPRIM IC50 = 1.0 nM 9.00