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Assay Detail

CHEMBL5162230

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human CXCR2 expressed in human U87 cells assessed as inhibition of CXCL8 AF647-binding incubated for 15 mins followed by addition of CXCL8 AF647 measured after 30 mins by flow cytometry analysis
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type U87
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

C-X-C chemokine receptor type 2 (CHEMBL2434)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of novel chemotypes as CXCR2 antagonists via a scaffold hopping approach from a thiazolo[4,5-d]pyrimidine.
Van Hoof M, Boon K, Van Loy T, Schols D, Dehaen W, De Jonghe S.
Eur J Med Chem (2022) 235 : 114268 -114268
PubMed 35313168 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.47 7.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL397237 1 7.89
CHEMBL5195797 1 7.89
CHEMBL5173775 1 7.36
CHEMBL5186932 1 6.89
CHEMBL5203743 1 6.60
CHEMBL5183834 1 6.37
CHEMBL5182926 1 5.97
CHEMBL5179622 1 5.57
CHEMBL5171358 1 5.37
CHEMBL5179345 1 4.76
CHEMBL5183117 1 -
CHEMBL5199496 1 -
CHEMBL5203131 1 -
CHEMBL5184465 1 -
CHEMBL5174137 1 -
CHEMBL5201451 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL397237 IC50 = 13.0 nM 7.89
CHEMBL5195797 IC50 = 13.0 nM 7.89
CHEMBL5173775 IC50 = 44.0 nM 7.36
CHEMBL5186932 IC50 = 130.0 nM 6.89
CHEMBL5203743 IC50 = 250.0 nM 6.60
CHEMBL5183834 IC50 = 430.0 nM 6.37
CHEMBL5182926 IC50 = 1060.0 nM 5.97
CHEMBL5179622 IC50 = 2710.0 nM 5.57
CHEMBL5171358 IC50 = 4260.0 nM 5.37
CHEMBL5179345 IC50 = 17170.0 nM 4.76
CHEMBL5183117 IC50 > 30000.0 nM -
CHEMBL5199496 IC50 > 30000.0 nM -
CHEMBL5203131 IC50 > 30000.0 nM -
CHEMBL5184465 IC50 > 30000.0 nM -
CHEMBL5174137 IC50 > 30000.0 nM -
CHEMBL5201451 IC50 > 30000.0 nM -