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Assay Detail

CHEMBL5162400

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at yeast Gal4-fused human PPARdelta transfected in human HepG2 cells assessed as transactivation by measuring beta-galactosidase activity incubated for 20 hrs by luminometry
26
Total Activities
26
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HepG2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor delta (CHEMBL3979)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A chemoinformatics search for peroxisome proliferator-activated receptors ligands revealed a new pan-agonist able to reduce lipid accumulation and improve insulin sensitivity.
Sblano S, Cerchia C, Laghezza A, Piemontese L, Brunetti L, Leuci R, Gilardi F, Thomas A, Genovese M, Santi A, Tortorella P, Paoli P, Lavecchia A, Loiodice F.
Eur J Med Chem (2022) 235 : 114240 -114240
PubMed 35325635 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Activity 23 - -
EC50 3 5.55 7.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL153057 1 7.68
CHEMBL5195064 1 4.55
CHEMBL1967191 1 4.43
CHEMBL5184438 1 -
CHEMBL5207420 1 -
CHEMBL5196830 1 -
CHEMBL5171340 1 -
CHEMBL5204992 1 -
CHEMBL5173134 1 -
CHEMBL5191690 1 -
CHEMBL5194126 1 -
CHEMBL5191536 1 -
CHEMBL5207071 1 -
CHEMBL5203327 1 -
CHEMBL5172173 1 -
CHEMBL5199782 1 -
CHEMBL5186824 1 -
CHEMBL5194570 1 -
CHEMBL5173310 1 -
CHEMBL5190696 1 -
CHEMBL5171949 1 -
CHEMBL5196394 1 -
CHEMBL5173673 1 -
CHEMBL5193822 1 -
CHEMBL295416 PIRINIXIC ACID 2.0 1 -
CHEMBL121 ROSIGLITAZONE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL153057 EC50 = 21.0 nM 7.68
CHEMBL5195064 EC50 = 28000.0 nM 4.55
CHEMBL1967191 EC50 = 37100.0 nM 4.43
CHEMBL5184438 Activity - -
CHEMBL5207420 Activity - -
CHEMBL5196830 Activity - -
CHEMBL5171340 Activity - -
CHEMBL5204992 Activity - -
CHEMBL5173134 Activity - -
CHEMBL5191690 Activity - -
CHEMBL5194126 Activity - -
CHEMBL5191536 Activity - -
CHEMBL5207071 Activity - -
CHEMBL5203327 Activity - -
CHEMBL5172173 Activity - -
CHEMBL5199782 Activity - -
CHEMBL5186824 Activity - -
CHEMBL5194570 Activity - -
CHEMBL5173310 Activity - -
CHEMBL5190696 Activity - -
CHEMBL5171949 Activity - -
CHEMBL5196394 Activity - -
CHEMBL5173673 Activity - -
CHEMBL5193822 Activity - -
CHEMBL295416 PIRINIXIC ACID Activity - -
CHEMBL121 ROSIGLITAZONE Activity - -