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Assay Detail

CHEMBL5162525

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of C-terminal His6-tagged BRD3 BD2 (unknown origin) using H-YSGRGK(Ac)GGK(Ac)GLGK(Ac)-GGAK(Ac)RHRK-Biotin-OH as substrate incubated for 1 hrs by HTRF assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 3 (CHEMBL1795186)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Discovery and Optimization of Furo[3,2-<i>c</i>]pyridin-4(5<i>H</i>)-one Derivatives as Potent and Second Bromodomain (BD2)-Selective Bromo and Extra Terminal Domain (BET) Inhibitors.
Li J, Zhang C, Xu H, Wang C, Dong R, Shen H, Zhuang X, Chen X, Li Q, Lu J, Zhang M, Wu X, Loomes KM, Zhou Y, Zhang Y, Liu J, Xu Y.
J Med Chem (2022) 65 : 5760 -5799
PubMed 35333526 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 8.39 9.06

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4297454 ABBV-744 1.0 1 9.06
CHEMBL1957266 1 8.31
CHEMBL5206088 1 8.28
CHEMBL5204783 1 7.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4297454 ABBV-744 IC50 = 0.88 nM 9.06
CHEMBL1957266 IC50 = 4.87 nM 8.31
CHEMBL5206088 IC50 = 5.31 nM 8.28
CHEMBL5204783 IC50 = 12.81 nM 7.89